甲异靛 - CAS:97207-47-1 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	甲异靛
英文名称:	Meisoindigo
别名:	甲异靛 N-Methylisoindigotin;Dian III;(E)-1,1'-dimethyl-[3,3'-biindolinylidene]-2,2'-dione
CAS No:	97207-47-1	分子式:	C₁₈H₁₄N₂O₂	分子量:	290.31
CAS No:	97207-47-1
分子式:	C₁₈H₁₄N₂O₂
分子量:	290.31

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M10320 甲异靛 ≥98%(HPLC) (psaitong)

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包装规格：

100mg 500mg in glass bottle

溶解性：

溶于DMSO(≥50mg/mL)

产品描述：

基本信息

产品编号：M10320
产品名称：Meisoindigo
CAS:	97207-47-1	储存条件	粉末	-20℃	四年

分子式：	C18H14N2O2		溶于液体	-80℃	六个月
分子量	290.31			-20℃	一个月
化学名：
Solubility (25°C)	体外	DMSO	55mg/mL (199.06mM)
		Ethanol	1mg/mL (3.61mM)
		Water	Insoluble
	体内（现配现用）
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	3.6194mL	18.0969mL	36.1939mL
5mM	0.7239mL	3.6194mL	7.2388mL
10mM	0.3619mL	1.8097mL	3.6194mL
50mM	0.0724mL	0.3619mL	0.7239mL

生物活性

产品描述	一种可诱导急性髓性白血病的凋亡和髓样分化的化合物。
靶点/IC50	Apoptosis
体外研究	Meisoindigo (Dian III; 5-20μM; for 24 hours) inhibits growth of the AML cell lines.Meisoindigo (10μM; for 24 hours) induces apoptosis of acute myeloid leukemia.Meisoindigo (5-10μM; for 24hours) causes cell-cycle arrest.Meisoindigo (5-10μM; for 24 hours) increases the cleaved caspase-3 and pro-apoptotic Bak, and decreases Bcl-2 and Bcl-xL levels in HL-60 cells.Meisoindigo (10, 30, 50, 100, 150μM; 24 hours) interdicts LPS-induced (1μg/mL) NLRP3 inflammasome activation and M1/M2 polarization through down-regulation of TLR4 pathways after OGD/R in HT-22 and BV2 cells. Cell Viability Assay
	Cell Line:	HL-60, NB4, U937 leukemic cell lines
	Concentration:	5, 10, 15, 20μM
	Incubation Time:	For 24 hours
	Result:	Inhibited growth of the AML cell lines in a dose- and time-dependent manner.
	Apoptosis Analysis
	Cell Line:	HL-60 cells
	Concentration:	10μM
	Incubation Time:	For 24 hours
	Result:	Induced apoptosis of acute myeloid leukemia.
	Cell Cycle Analysis
	Cell Line:	HL-60 cells
	Concentration:	5, 10μM
	Incubation Time:	For 24 hours
	Result:	Caused cell-cycle arrest, with more cells in sub-G1 and G0/G1 phases and fewer cells in the S phase, in a dose-dependent manner.
	Western Blot Analysis
	Cell Line:	HL-60 cells
	Concentration:	5, 10μM
	Incubation Time:	For 24 hours
	Result:	Increased the cleaved caspase-3 and pro-apoptotic Bak, and decreased Bcl-2 and Bcl-xL levels in HL-60 cells.
体内研究	Meisoindigo (Dian III; 50-150mg/kg; IP; daily; for 14 days) has anti-leukemic activity in vivo.Meisoindigo (2, 4, 8, 12mg/kg; IP; before MCAO and 2h after reperfusion) significantly reduces infarct volume, ameliorates neurological deficits 3 days after middle cerebral artery occlusion (MCAO) in Wild-type C57BL/6J mice (25-30g).Meisoindigo reduces edema and lowers AQP4 expression in the brain.
	Animal Model:	NOD/SCID mice, 6-8 weeks old, with HL-60 leukemic cells
	Dosage:	50, 100, 150mg/kg
	Administration:	IP; daily; for 14 days
	Result:	Had anti-leukemic activity in vivo.