中文名称: | MDL 29951 | ||||
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英文名称: | MDL 29951 | ||||
别名: | MDL 29951 MDL29951;2-Carboxy-4,6-dichloro-1H-indole-3-propanoic acid | ||||
CAS No: | 130798-51-5 | 分子式: | C12H9Cl2NO4 | 分子量: | 302.11 |
CAS No: | 130798-51-5 | ||||
分子式: | C12H9Cl2NO4 | ||||
分子量: | 302.11 |
基本信息
产品编号: |
M10316 |
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产品名称: |
MDL 29951 |
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CAS: |
130798-51-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
6个月 |
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分子量: |
302.11 |
-20℃ |
1个月 |
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化学名: |
2-Carboxy-4,6-dichloro-1H-indole-3-propanoic acid |
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Solubility (25°C): |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
3.3101mL |
16.5503mL |
33.1005mL |
5mM |
0.6620mL |
3.3101mL |
6.6201mL |
10mM |
0.3310mL |
1.6550mL |
3.3101mL |
生物活性
产品描述 |
一种nMDA receptor拮抗剂,可抑制体内体外甘氨酸([3H]glycine)的结合,Ki值为0.14μM。 |
靶点 |
Ki:0.14μM |
体外研究 |
MDL 100,748 and MDL 29,951 are approximately 2000-fold selective for the glycine binding site relative to the glutamate recognition sites.MDL-29951 is found to inhibit the human F16Bpase under these conditions (IC50=2.5μM).MDL-29951 inhibits the human liver (IC50=2.5μM),porcine kidney (IC50=1.0μM),and rabbit liver (IC50=0.21μM) isoforms of the enzyme,but is significantly less potent against the rat liver isoform (IC50=11μM).The MDL29951-activated receptor exhibits other activities associated with GPCR-mediated signaling, including G protein-dependent activation of extracellular signalregulated kinase 1 and 2 (ERK1/2) and recruitment of β-arrestin.As with recombinant cell systems,MDL29951 promotes Ca2+ signaling responses and inhibition of cyclic adenosine monophosphate (cAMP) accumulation in rat oligodendrocyte precursor cells during the period of peak GPR17 abundance.Effects of MDL29951 are markedly reduced in cells with low GPR17 abundance and are blocked by pranlukast. |
推荐实验方法(仅供参考)
激酶实验: |
[3H]JCPP (30.7 Ci/mmol) binding assays are conducted in minivials,incubated for 15mm at 25℃ in 1mL of 50mM Tris-HC1 (pH 7.4) containing 10nM [3H]JCPP,200g of membrane protein and unlabeled ligands as indicated.Nonspecific binding is defined using 1mM L-glutamate.Bound ligand is separated by centrifugation. Specific binding accounted for approximately 80% of total binding. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )