JW55 - CAS:664993-53-7 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	JW55
英文名称:	JW55
别名:	JW55 JW-55; N-(4-(((4-(4-methoxyphenyl)tetrahydro-2H-pyran-4-yl)methyl)carbamoyl)phenyl)furan-2-carboxamide
CAS No:	664993-53-7	分子式:	C₂₅H₂₆N₂O₅	分子量:	434.48
CAS No:	664993-53-7
分子式:	C₂₅H₂₆N₂O₅
分子量:	434.48

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF)

J10023 JW55 ≥98%(HPLC) (psaitong)

相关产品

包装规格：

5mg 25mg 100mg in glass bottle

溶解性：

溶于DMSO(≥50mg/mL)

产品描述：

基本信息

产品编号：J10023
产品名称：JW55
CAS:	664993-53-7	储存条件	粉末	-20℃	四年

分子式：	C25H26N2O5		溶于液体	-80℃	两年
分子量	434.48			-20℃	一个月
化学名：	N-(4-(((4-(4-methoxyphenyl)tetrahydro-2H-pyran-4-yl)methyl)carbamoyl)phenyl)furan-2-carboxamide
Solubility (25°C)	体外	DMSO	87mg/mL (200.23mM)
		Ethanol	4mg/mL (9.2mM)
		Water	Insoluble
	体内	现配现用
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.3015mL	11.5077mL	23.0155mL
5mM	0.4603mL	2.3015mL	4.6031mL
10mM	0.2302mL	1.1508mL	2.3015mL
50mM	0.0460mL	0.2302mL	0.4603mL

生物活性

产品描述

一种有效的选择性β-catenin抑制剂，通过抑制端锚聚合酶(TNKS1/2)发挥作用。

靶点/IC₅₀

TNKS2

0.83μM (IC50)

TNKS1

1.9μM (IC50)

体外研究

JW 55 (JW55) is a potent and selective inhibitor of the canonical Wnt pathway. Wnt3a-induced HEK293 cells containing a transiently transfected ST-Luc (SuperTop-luciferase) reporter show inhibition by JW55 with an IC50 value of 470 nM. JW55 is effective in the range of 1 to 5μM in SW480 cells and 0.01 to 5μM in HCT-15 cells. JW55 is effective in the range of 1 to 5μM in

SW480 cells and 0.01 to 5μM in HCT-15 cells.

体内研究

JW 55 (100mg/kg, orally) reduces tumor development in conditional Apc knockout mice. JW55 reduces XWnt8-induced axis duplication inXenopus embryos and Tamoxifen-induced polyposis formation in conditional APC mutant mice.

推荐实验方法(仅供参考)

细胞实验：

Cell Assay

A total of 1,000 SW480 or RKO cells are seeded in 96-well plates. The day after, the cell culture medium is exchanged to solutions that contained 0.1% DMSO or 10μM JW55 for RKO cells and 0.1% DMSO or 10, 5, or 1μM JW55 for SW480 cells. All samples consist of a minimum of 6 replicates. The plate is incubated in an IncuCyte inside a cell culture incubator. Images are captured every second hour to monitor proliferation.

动物实验:

Animal Administration

Mice

Seven 12-week old female ApcCKO/CKO/Lgr5-CreERT2 mice are injected intraperitonally with 25mg/kg of Tamoxifen diluted in an ethanol and corn oil (ratio 1:4). The mice are randomized into 2 groups and treated with either JW55 (100 mg/kg) or vehicle (DMSO). Daily per oral applications started the day after and continued for 3 weeks. The mouse body weight is measured twice a week. The mice are sacrificed and the intestines are dissected, washed in PBS, and fixed in formaldehyde [10% solution (v/v) in PBS]. The small intestines are stained using 1% methylene blue prepared in 10% paraformalaldehyde (PFA)/PBS solution. Small ileum Swiss-rolls are embedded in paraffin sectioned and stained with hematoxylin and eosin. Fixed colons are embedded in paraffin, sectioned and stained with an anti-β-catenin antibody. The number and size of the intestinal lesions are quantified by the Ellipse program.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

搜索质检报告(COA)

参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果