Inauhzin - CAS:309271-94-1 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	Inauhzin
英文名称:	Inauhzin
别名:	Inauhzin 2-((5H-[1,2,4]triazino[5,6-b]indol-3-yl)thio)-1-(10H-phenothiazin-10-yl)butan-1-one
CAS No:	309271-94-1	分子式:	C₂₅H₁₉N₅Os₂	分子量:	469.58
CAS No:	309271-94-1
分子式:	C₂₅H₁₉N₅Os₂
分子量:	469.58

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF)

I10168 Inauhzin ≥98%(HPLC) (psaitong)

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包装规格：

5mg 25mg 100mg in glass bottle

溶解性：

溶于DMSO(20mg/mL 加热超声)

产品描述：

基本信息

产品编号：I10168
产品名称：Inauhzin
CAS:	309271-94-1	储存条件	粉末	-20℃	四年

分子式：	C25H19N5Os2		溶于液体	-80℃	两年
分子量	469.58			-20℃	一个月
化学名：	2-((5H-[1,2,4]triazino[5,6-b]indol-3-yl)thio)-1-(10H-phenothiazin-10-yl)butan-1-one
Solubility (25°C)	体外	DMSO	94mg/mL (200.17mM)
		Ethanol	Insoluble
		Water	Insoluble
	体内	现配现用
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.1296mL	10.6478mL	21.2956mL
5mM	0.4259mL	2.1296mL	4.2591mL
10mM	0.2130mL	1.0648mL	2.1296mL
50mM	0.0426mL	0.2130mL	0.4259mL

生物活性

产品描述	一种SirT1/IMPDH2的双重抑制剂，同时为p53的激活剂。
靶点/IC₅₀	SIRT1 ()	p53 ()
体外研究	Inauhzin (10µM) induces p53 levels as effectively as actinomycin D (10nM), and mediates p53-dependent cytotoxicity through its specific functional groups in human lung carcinoma H460 cells. Inauhzin (2µM) induces p53 level and activity as well as p53-dependent apoptosis. Inauhzin also stabilizes p53 and inhibits its ubiquitylation. Inauhzin induces acetylation of p53 in H460 cells, but not tubulin, which is affected by knockdown of SIRT1. Inauhzin (0-2 µM) significantly enhances the expression level and activity of p53 in HCT116^p53+/+ cells and enhances the expression level and activity of p53 in H460 cells in a dose-dependent manner. Inauhzin and Nutlin-3 demonstrate synergistic cytotoxicity in the Nutlin-3 low-sensitive cells. Inauhzin and Nutlin-3 synergistically induce p53-dependent apoptosis. Inauhzin targets both SirT1 and IMP dehydrogenase 2 (IMPDH2), and acts as a potent p53 activator.
体内研究	Inauhzin (30mg/kg, i.p.) effectively induces apoptosis and suppresses tumour growth of H460 xenograft harbouring p53. Inauhzin (30mg/kg, i.p.) reduces the HCT116 tumor volume by appr 70%. Inauhzin (15mg/kg) in combination with 150mg/kg of Nutlin-3 demonstrates a significant synergy on p53 induction, apoptosis and tumor suppression of HCT116^p53+/+ xenografts.

推荐实验方法(仅供参考)

细胞实验：

Cell Assay

The cell counting kit is used to assess cell growth. Cell suspensions are seeded at 5000 cells per well in 96-well culture plates and incubated overnight at 37°C. Compounds are added into the plates and incubated at 37°C for 72 h. Cell growth inhibition is determined by adding WST-8 at a final concentration of 10% to each well, and the absorbance of the samples is measured at 450nm using a Microplate Reader.

动物实验:

Animal Administration

Five-weeks-old female SCID mice are housed in a BSL2 environment. Mice are subcutaneously inoculated with 5×10⁶ H460 or 3×10⁶ HCT116 cells. Tumour growth is monitored every other day with electronic digital calipers in two dimensions.Tumour volume is calculated with the formula: tumour volume (mm³) = (length × width²)/2. When the mean tumour volume reaches approximately 100 mm³ after 7-9 days, animals are dosed by i.p. injection with vehicle (5% DMSO) or Inauhzin. Inhibition of tumour growth is calculated on the last day of treatment. To detect p53 activation in vivo, tumours are harvested and disrupted in RIPA buffer containing a protease inhibitor mixture. Tumour lysates are analysed by IB. Cell proliferation in tumours is assessed by BrdU labeling followed by Immunostaining. 200mg/kg body weight of BrdU is administrated to mice via i.p. injection 2 h before mice are sacrificed. Apoptosis is examined by TUNEL staining, using the Fluorescein In situ cell death detection kit

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

搜索质检报告(COA)

参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果