18α-甘草次酸 - CAS:1449-05-4 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	18α-甘草次酸
英文名称:	18α-glycyrrhetinic acid
别名:	甘草次酸（α型） 20β-olean-12-en-29-oic acid
CAS No:	1449-05-4	分子式:	C₃₀H₄₆O₄	分子量:	470.68
CAS No:	1449-05-4
分子式:	C₃₀H₄₆O₄
分子量:	470.68
MDL:	MFCD00064897	EINEC:	215-907-9
EINEC:	215-907-9

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G10630 18α-甘草次酸分析标准品 (psaitong)

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包装规格：

20mg in glass bottle

溶解性：

溶于DMSO(10mg/ml 超声）

产品描述：

基本信息

产品编号：	G10630
产品名称：	18α-glycyrrhetinic acid
CAS:	1449-05-4	储存条件	粉末	-20℃	四年

分子式：	C30H46O4		溶于液体	-80℃	6个月
分子量：	470.68			-20℃	1个月
化学名：	20β-olean-12-en-29-oic acid
Solubility (25°C)：
体外:	DMSO	94mg/mL (199.71mM)
	Ethanol	46mg/mL (97.73mM)
	Water	Insoluble
体内(现配现用):	1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:1.92mg/mL(4.08mM);Suspended solution;Need ultrasonic
	此⽅案可获得 1.92 mg/mL (4.08 mM) 的均匀悬浊液，悬浊液可⽤于⼝服和腹腔注射。以 1 mL ⼯作液为例，取 100 μL 19.199999 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加⼊ 50 μL Tween-80，混合均匀；然后继续加⼊ 450 μL ⽣理盐⽔定容⾄ 1 mL。
	2.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥1.92mg/mL(4.08mM);Clear solution
	此⽅案可获得 ≥ 1.92 mg/mL (4.08 mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1 mL ⼯作液为例，取 100 μL 19.199999 mg/mL 的澄清 DMSO 储备液加到 900 μL ⽟⽶油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.1246mL	10.6229mL	21.2459mL
5mM	0.4249mL	2.1246mL	4.2492mL
10mM	0.2125mL	1.0623mL	2.1246mL

生物活性

产品描述	一种饮食衍生的化合物，是NF-kB的抑制剂和蛋白酶体(proteasome)的激活剂，在多细胞生物中起到促进长寿和抗聚集因子的作用。18α-Glycyrrhetinic acid诱导细胞凋亡。
靶点	NF-κB	Proteasome
体外研究	18α-Glycyrrhetinic acid (18a-GA) markedly reduces LX-2 cell numbers by 14.8% and 31.2% after 48h and 72h of treatment,respectively (P< 0.05).18α-Glycyrrhetinic acid also significantly increases the percentage of LX-2 cells in phase G0/G1 and decreases it in phase S after treated for 48h and 72h compare with the control group.18α-Glycyrrhetinic acid increases apoptosis to 6.8% at 48h,compare with control (2.5%),and at 72h the percentages of apoptotic cells in control and the treatment groups are 3.1% and 15.6%,respectively,in LX-2 cells (P<0.01).Furthermore,18α-Glycyrrhetinic acid induces expression of PPAR-γ and alters some cell cycle and apoptosis-related proteins.18α-Glycyrrhetinic acid also inhibits NF-κB DNA-binding activity.
体内研究	18α-Glycyrrhetinic acid (18α-GA) treatment significantly enhances life span of C.elegans strains with the most effective concentration being 20μg/mL.Results reveal a significant delay of paralysis upon 18α-Glycyrrhetinic acid treatment.18αGlycyrrhetinic acid treatment also confers a significant reduction of Aβ deposits.

推荐实验方法(仅供参考)

细胞实验:

For the determination of cell cycle,5×105 cells per well are seeded onto 6-well plates and incubated overnight in complete growth medium (DMEM+10% FBS).After starvation for 24h,the cells are subsequently stimulated with 10% FBS with or without the presence of 18α-Glycyrrhetinic acid (18a-GA).The final concentration of 18α-Glycyrrhetinic acid is 8.0mM.The cells are incubated for 24h,48 h and 72h,respectively,and then the flow cytometric analysis is performed.Flow cytometric analysis is performed in triplicate.After being treated with 18α-Glycyrrhetinic acid,the cells are stained and then cell apoptosis status is measured by flow cytometry.

动物实验:

C.elegans strains are used in this study.Synchronized L4 larvae CL2006 animals (100 to 120 animals per condition) are transferred to nematode growth medium (NGM) plates containing either 18α-Glycyrrhetinic acid (18α-GA) or DMSO at 20℃.Synchronized CL4176 animals (150 to 300 animals per condition) are transferred to NGM plates containing either 18αGlycyrrhetinic acid or DMSO at 16℃ for 48h before temperature upshift to 25℃ for transgene induction.Scoring of paralyzed animals is initiated at day 1 of adulthood for CL2006 strain and 24h after temperature upshift for CL4176 strain.Each paralysis assay is repeated at least thrice.Nematodes are scored as paralyzed if they exhibit halos of cleared bacteria around their heads or fail to undergo half end body wave propagation upon prodding.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果