中文名称: | Galiellalactone | ||||
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英文名称: | Galiellalactone | ||||
别名: | Galiellalactone Galiellalactone | ||||
CAS No: | 133613-71-5 | 分子式: | C11H14O3 | 分子量: | 194.23 |
CAS No: | 133613-71-5 | ||||
分子式: | C11H14O3 | ||||
分子量: | 194.23 |
基本信息
产品编号: |
G10553 |
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产品名称: |
Galiellalactone |
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CAS: |
133613-71-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
分子式: |
溶于液体 |
-80℃ |
二年 |
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分子量: |
194.23 |
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化学名: |
(4R,7R,9S,11S)-11-Hydroxy-9-methyl-3-oxatricyclo[5.3.1.04,11]undec-1(10)-en-2-one |
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Solubility (25°C): |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
生物活性
产品描述 |
一种小的无毒且无致突变性的真菌代谢产物,是一种选择性的STAT3信号抑制剂,IC50值为250-500nM。Galiellalactone可用于研究去势抵抗性前列腺癌。 |
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靶点 |
STAT3 250-500nM (IC50) |
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体外研究 |
Galiellalactone (2.5-25μM;24-72 hour) induces apoptosis of prostate cancer (PCa) cell lines expressingp-Stat3.Galiellalactone (2.5-25μM;24-72 hour) decreases viability of DU145 cells.Galiellalactone (10μM) inhibits STAT3 signaling activity as assessed by luciferase reporter gene assay in IL-6-stimulated LNCaP cells.Galiellalactone (10-100μM;1 hour) binds directly to STAT3 in DU145 cells.Galiellalactone (5-50μM;1 hour) interferes with STAT3 DNA binding without inhibiting phosphorylation in DU145 cells. Apoptosis Analysis |
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Cell Line: |
DU145,PC-3,and LNCaP cells |
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Concentration: |
0,2.5,5,10,25μM |
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Incubation Time: |
24,48,72 hours |
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Result: |
Induced an apoptotic response in PC-3 and DU145 cells. |
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Cell Viability Assay |
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Cell Line: |
DU145 cells |
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Concentration: |
0,2.5,5,10,17.5,25μM |
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Incubation Time: |
24,48,72 hours |
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Result: |
Decreased DU145 cells viability in a dose-and time-dependent manner. |
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Western Blot Analysis |
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Cell Line: |
DU145 cells |
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Concentration: |
0,10,25μM |
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Incubation Time: |
48 hours |
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Result: |
Induced expression of the apoptotic marker cleaved caspase-3 and increased expression of cleaved PARP. |
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Western Blot Analysis |
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Cell Line: |
DU145 cells |
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Concentration: |
0,10,25,50,100μM |
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Incubation Time: |
1 hour |
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Result: |
Prevented the binding of the biotinylated analogue of galiellalactone (GL-biot) to STAT3 in a dose-dependent manner demonstrating competitive binding. |
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Western Blot Analysis |
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Cell Line: |
DU145 cells |
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Concentration: |
0,5,10,25,50μM |
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Incubation Time: |
1 hour |
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Result: |
Inhibited STAT3 binding to DNA in a dose-dependent manner.Did not affect phosphorylation of STAT3 Tyr-705 and Ser-727 or the expression of total STAT3. |
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体内研究 |
Galiellalactone (1,3mg/kg;daily i.p.for 3 weeks) inhibits PCa tumor growth in vivo. |
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Animal Model: |
Male nude NMR1 mice are injected PCa cell |
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Dosage: |
0,1,3mg/kg |
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Administration: |
Daily i.p.injections for 3 weeks |
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Result: |
Reduced the tumor growth rate in DU145 xenografts by 41-42% and was well tolerated. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )