EB-3D - CAS:1839150-63-8 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	EB-3D
英文名称:	EB-3D
别名:	EB-3D;1,1'-(((ethane-1,2-diylbis(oxy))bis(4,1-phenylene))bis(methylene))bis(4-(dimethylamino)pyridinium) bromide;1-[[4-[2-[4-[[4-(Dimethylamino)pyridin-1-ium-1-yl]methyl]phenoxy]ethoxy]phenyl]methyl]-N,N-dimethylp
CAS No:	1839150-63-8	分子式:	C₃₀H₃₆Br₂N₄O₂	分子量:	644.44
CAS No:	1839150-63-8
分子式:	C₃₀H₃₆Br₂N₄O₂
分子量:	644.44

安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF)

E10344 EB-3D ≥98% (psaitong)

相关产品

包装规格：

5mg 10mg 50mg 100mg in glass bottle

溶解性：

溶于DMSO（50 mg/mL 超声）

产品描述：

基本信息

产品编号：E10344
产品名称：EB-3D
CAS:	1839150-63-8	储存条件	粉末	-20℃	四年

分子式：	C30H36Br2N4O2		溶于液体	-80℃	六个月
分子量：	644.44			-20℃	一个月
化学名：
Solubility (25°C)	体外	DMSO	50mg/mL (77.58mM)
		Ethanol	15mg/mL (23.27mM)
		Water	25mg/mL (38.79mM)
	体内（现配现用）
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	1.5517mL	7.7587mL	15.5173mL
5mM	0.3103mL	1.5517mL	3.1035mL
10mM	0.1552mL	0.7759mL	1.5517mL
50mM	0.0310mL	0.1552mL	0.3103mL

生物活性

产品描述	是有效的选择性胆碱激酶 α (ChoKα) 抑制剂，对 ChoKα1 的 IC50 值为 1μM。
靶点/IC50	ChoKα1 (Cell-free assay)
靶点/IC50	1μM
体外研究	EB-3D displays (0-100μM;72 hours) excellent antiproliferative activity against a wide cohort of T-leukemic cell lines,with GI GI50s 13 values in the nanomolar range.EB-3D (1.25-5μM;24 hours) induced apoptosis in leukemia cell lines.EB-3D (0.5-1μM;24 hours) induces a G0/G1 arrest that lead to apoptosis.EB-3D (0.3μM;48 hours) shows a first spike of activation of AMPKα after 30 minutes and a later increase in the phosphorylation of T172.EB-3D (1-40μM;48 hours) inhibits cell growth in HepG2 cells with a GI50 of 14.55μM.EB-3D induces deregulation of the AMPK-mTOR pathway and apoptosis in leukemia T-cells. Cell Proliferation Assay
	Cell Line:	JURKAT,CCRF-CEM,HSB-2,MOLT-16,DNA-41,LOUCY,PEER,ALL-SIL cells
	Concentration:	0.001,0.01,0.1,1,10,100μM
	Incubation Time:	72 hours
	Result:	Inhibited JURKAT,CCRF-CEM,HSB-2,MOLT-16,DNA-41,LOUCY,PEER,and ALL-SIL cells growth with GI50s of 136.2,478.8,17.7,0.9,60.6,200,265,and 132nM,respectively.
	Apoptosis Analysis
	Cell Line:	Jurkat, CCRF-CEM and HSB-2 cells
	Concentration:	1.25,2.5,5μM
	Incubation Time:	24 hours
	Result:	Induced apoptosis in leukemia cell lines.
	Cell Cycle Analysis
	Cell Line:	Jurkat, CCRF-CEM and HSB-2 cells
	Concentration:	0.5,1μM
	Incubation Time:	24 hours
	Result:	Induces cell cycle arrest in G0/G1 phase.
	Western Blot Analysis
	Cell Line:	Jurkat cells
	Concentration:	0.3μM
	Incubation Time:	48 hours
	Result:	Showed a first spike of activation of AMPKα after 30 minutes of treatment and a later increase in the phosphorylation of T172.The increase in S79 phosphorylation of its main target ACC (acetyl-coenzyme A (CoA) carboxylase),followed the same pattern.This rapid activation of AMPK,in turn induced a consequent reduction in mTOR phosphorylation that is visible already at 30’and that becomes amplified at longer time probably due to the interruption of feedback loops that are characteristic of mTOR connecting pathways.
体内研究	EB-3D (1mg/kg;i.p.;every other day) impairs mammary tumor growth in syngeneic orthotopic E0771-C57BL/6 mouse model.EB-3D (2.5mg/kg; every other day for 4 weeks) shows a reduction of the number of spontaneous lung macro- and micrometastasis.
	Animal Model:	E0771-C57BL/6 mice
	Dosage:	I.p.;every other day for 4 weeks
	Administration:	2.5mg/kg
	Result:	A reduction of the number of spontaneous lung macro-and micrometastasis.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

搜索质检报告(COA)

参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果