厄多司坦 - CAS:84611-23-4 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	厄多司坦
英文名称:	Erdosteine
别名:	厄多司坦 RV 144
CAS No:	84611-23-4	分子式:	C₈H₁₁NO₄S₂	分子量:	249.31
CAS No:	84611-23-4
分子式:	C₈H₁₁NO₄S₂
分子量:	249.31

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E10297 厄多司坦 ≥98% (psaitong)

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包装规格：

1g 5g in glass bottle

溶解性：

溶于DMSO（50 mg/mL超声)

产品描述：

基本信息

产品编号：	E10297
产品名称：	Erdosteine
CAS:	84611-23-4	储存条件	粉末	-20℃	四年

分子式：	C8H11NO4S2		溶于液体	-80℃	6个月
分子量：	249.31			-20℃	1个月
化学名：	2-((2-Oxo-2-((2-oxotetrahydrothiophen-3-yl)amino)ethyl)thio)acetic acid
Solubility (25°C)：
体外:	DMSO	50mg/mL (200.55mM)
	Ethanol	10mg/mL (40.11mM)
	Water	Insoluble
体内(现配现用):	1.请依序添加每种溶剂：PBS Solubility:6.67mg/mL(26.75mM);Clear solution;Need ultrasonic
	2.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥3.25mg/mL(13.04mM);Clear solution
	此⽅案可获得 ≥ 3.25 mg/mL (13.04 mM，饱和度未知) 的澄清溶液。以 1 mL ⼯作液为例，取 100 μL 32.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加⼊ 50 μ L Tween-80，混合均匀；然后继续加⼊ 450 μL ⽣理盐⽔定容⾄ 1 mL。
	3.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility:≥3.25mg/mL(13.04mM);Clear solution
	此⽅案可获得 ≥ 3.25 mg/mL (13.04 mM，饱和度未知) 的澄清溶液。以 1 mL ⼯作液为例，取 100 μL 32.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。
	4.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥3.25mg/mL(13.04mM);Clear solution
	此⽅案可获得 ≥ 3.25 mg/mL (13.04 mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1 mL ⼯作液为例，取 100 μL 32.5 mg/mL 的澄清 DMSO 储备液加到 900 μL ⽟⽶油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	4.0111mL	20.0554mL	40.1107mL
5mM	0.8022mL	4.0111mL	8.0221mL
10mM	0.4011mL	2.0055mL	4.0111mL

生物活性

产品描述	抑制脂多糖(LPS)诱导的NF-κB激活,具有粘液调节，抗菌，抗炎和抗氧化作用。
靶点	NF-κB
体外研究	Erdosteine is an oral mucolytic agent used as an expectorant in various chronic respiratory diseases.Erdosteine exerts antiinflammatory effects by inhibiting NF-κB activation in LPS-stimulated mouse macrophages.However,Erdosteine does not inhibit LPS induced phosphorylation of the Akt and MAPK pathways.To evaluate the toxic effects of Erdosteine on macrophages, cell viability is analyzed.Treatment with 1,10,or 100μg/mL Erdosteine does not produce detectable cytotoxicity.Treatment with LPS (1μg/mL) induced IκBα degradation in RAW 264.7 cells,and maximal degradation is observed after 10 min.RAW 264.7 cells are pretreated with the indicated concentrations of Erdosteine for 6h and then stimulated with LPS (1μg/mL) for 10 min.Pretreatment with Erdosteine does not have any effect on the baseline amount of I κBα.Treatment with DMSO alone at a volume equal to that used for Erdosteine delivery does not have any effect on the baseline amount of IκBα.The amount of IκBα is decreased by treatment with LPS for 10 min,and pretreatment with Erdosteine at the indicated concentration and time effectively inhibits IκBα degradation.
体内研究	Twenty-six male mice are divided into four groups as follows:group 1,control;group 2,Erdosteine-treated;group 3,Methotrexate (MTX)-treated;and group 4,Methotrexate+Erdosteine treated.On the first day of experiment,a single dose of Methotrexate is intraperitoneally administered to groups 3 and 4,although a daily single dose of Erdosteine is orally administered to group 2 and 4 for 7 days.At the end of the experiment,the testes of the animals are removed and weighed.The levels of total antioxidant capacity and total oxidative stress, and myeloperoxidase activity in the Methotrexate group are higher than the control group (p<0.05).Lipid peroxidation levels are not changed in Methotrexate group compared with control group.In conclusion,Erdosteine can effectively protect the testes in Methotrexate-induced toxicity.Erdosteine administration with Methotrexate improves testicular injures,as indicated by appearance of spermatogenesis in seminiferous tubules.

推荐实验方法(仅供参考)

细胞实验:

The murine macrophage/monocyte cell line RAW264.7 are maintained as monolayers in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum,60 U/mL Penicillin,and 100μg/mL Streptomycin at 37.8℃ in 5% CO2.The cell viability is quantified using a colorimetric tetrazolium compound MTS assay.Briefly,1×104 cells incubated with various concentrations of Erdosteine (1,10,or 100μg/mL) for 24h are treated with 10μL of MTS solution (5mg/mL) for 45 min.The cells are then lysed with isopropyl alcohol,and the absorbance is read at the wavelength of 540nm.

动物实验:

Mice

Twenty-six male C57BL/6 mice (8 weeks,20-30g) are randomly divided into four groups. In control group (n=6);mice are treated the 0.5mL of saline as a placebo intraperitoneally (i.p.).In Erdosteine group (n=6),mice are treated with Erdosteine orally (gavage;10mg/kg) for 7 days.In this study,low-dose MTX are used because high-dose (20-40mg/kg) MTX has antiinflammatory and immunosuppressive activity.Mice in MTX group (n=7) are injected with single dose of i.p.MTX (10mg/kg).In MTX+Erdosteine group (n=7),mice are injected with single dose of i.p.MTX (10mg/kg) the first day and Erdosteine is given orally (10mg/kg) to the animals starting the first day for 7 days.After the last administration of the drug,all rats fasted about 12 hours,but have free access to water.And then,the animals are sacrificed by cervical dislocation at the end of the experiment.Following sacrifice,the testes are quickly removed from the mice.Right testes specimens are fixed in 10% neutral-buffered formaldehyde solution for histological assessment.

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

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