| 中文名称: | 2,6-二羟基黄酮 | ||||
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| 英文名称: | 2',6-Dihydroxyflavone | ||||
| 别名: | 6,2-二羟基黄酮 6,2'-Dihydroxyflavone | ||||
| CAS No: | 92439-20-8 | 分子式: | C15H10O4 | 分子量: | 254.24 |
| CAS No: | 92439-20-8 | ||||
| 分子式: | C15H10O4 | ||||
| 分子量: | 254.24 | ||||
基本信息
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产品编号： |
D11025 |
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产品名称： |
2',6-Dihydroxyflavone |
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CAS: |
92439-20-8 |
储存条件 |
粉末 |
2-8℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
254.24 |
-20℃ |
1个月 |
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化学名： |
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Solubility (25°C)： |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.9333mL |
19.6665mL |
39.3329mL |
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5mM |
0.7867mL |
3.9333mL |
7.8666mL |
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10mM |
0.3933mL |
1.9666mL |
3.9333mL |
生物活性
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产品描述 |
一种新型的GABAA受体拮抗剂。 |
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靶点 |
GABAA receptor |
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体外研究 |
6,2'-Dihydroxyflavone is a novel antagonist of GABAA receptor.6,2'-Dihydroxyflavone inhibits [3H]-flunitrazepam binding to the rat cerebral cortex membranes with a Ki of 37.2±4.5nM.The current elicited with the EC50 concentration of GABA is decreased to 73.6±1.9% of control by co-application of 5μM 6,2'-Dihydroxyflavone (n=5),compare to a decrease to 65.9±3.0% by 1μM FG-7142 (n=5).The EC50 for GABA dose response increases from 47.6 to 59.7μM upon co-application of 5μM 6,2'-Dihydroxyflavone,and the maximal GABA-current is decreased. |
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体内研究 |
6,2'-Dihydroxyflavone-treated mice exhibit significant differences from control mice with respect to the percentage of open arms entries [F(4,73)=8.01,P<0.0001] and the percentage of time spent in open arms [F (4,73)=5.19,P<0.002],but not the number of entries to closed arms [F(4,73)= 0.79,P=0.54].The post-hoc NewmaneKeuls’tests confirm that 6,2'-Dihydroxyflavone significantly decreases the percentage of open arm entries and time spent in open arms at the doses of 8 and 16mg/kg.6,2'-Dihydroxyflavone treatment similarly increases step-through latency [F(4,75)=4.71, P<0.002],suggesting enhanced cognitive performance. |
推荐实验方法(仅供参考)
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细胞实验: |
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Membranes from HEK 293T cell are used in this study.Briefly,aliquots of membranes are incubated with 1nM [3H]-flunitrazepam or 8nM [3H]-Ro15-4513 at 4℃ for 90 min in the presence or absence of 6,2'-Dihydroxyflavone. After incubation,the mixtures are filtered onto Whatman GF/B filters with a Brandel 24-well harvester. Each filter is incubated for at least 1h with 4mL scintillation cocktail before measurement of radioactivity in a Beckman-Coulter LS 6500 scintillation counter.For saturation analysis, the membranes are incubated with increasing concentrations of [3H]-flunitrazepam or [3H]-Ro15-4513.Binding affinity is determined by nonlinear regression analysis. |
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动物实验: |
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Male ICR mice are randomized into six groups (n=12 to 16/group), and receive 0.4mg/kg scopolamine (i.p.) 45 min prior to training,followed with vehicle (dd water,pH 9.0,p.o.),2,4,8 or 16mg/kg 6,2'-Dihydroxyflavone (p.o.),or 30mg/kg FG-7142 (i.p.) 30 min prior to training.On the training trials,each mouse is placed into the lighted chamber of a two-compartment box,and the door leading to the dark chamber is opened 10s later.Once the mouse enters the dark compartment,the door is closed and an inescapable electric foot-shock (0.4 mA,1s) is delivered from the grid floor.The mouse is removed from the apparatus 10s later.The step-through latency is recorded,with the cut-off step-through latency set at 300s. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
