细胞松弛素B - CAS:14930-96-2 - CAS号查询_生物试剂_化学试剂_分析试剂

中文名称:	细胞松弛素B 热销
英文名称:	Cytochalasin B
别名:	细胞分裂抑素B Cytochalasin B
CAS No:	14930-96-2	分子式:	C₂₉H₃₇NO₅	分子量:	479.61
CAS No:	14930-96-2
分子式:	C₂₉H₃₇NO₅
分子量:	479.61
MDL:	MFCD00077704	EINEC:	239-000-2
EINEC:	239-000-2

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C60031 细胞松弛素B ≥98% (psaitong)

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熔点：

215°C - 226°C

包装规格：

1mg 5mg 10mg in glass bottle

溶解性：

溶于DMSO和乙醇(10mg/mL)

产品描述：

基本信息

产品编号：	C60031
产品名称：	Cytochalasin B
CAS:	14930-96-2	储存条件	粉末	-20℃	四年

分子式：	C29H37NO5		溶于液体	-80℃	六个月
分子量	479.61			-20℃	一个月
化学名：	24-Oxa(14)cytochalasa-6(12),13,21-triene-1,23-dione, 7,20-dihydroxy-16-methyl-10-phenyl-, (7S,13E,16R,20R,21E)-
Solubility (25°C)：
体外:	DMSO	80mg/mL (166.8022mM; ultrasonic and warming and heat to 60°C)
	Ethanol	25mg/mL (52.13mM; Need ultrasonic)
	Water
体内(现配现用):	1.请依序添加每种溶剂：0.5% CMC-Na→0.5% Tween-80 Solubility: 5mg/mL (10.43mM); Suspened solution; Need ultrasonic
	2.请依序添加每种溶剂：10% EtOH→40% PEG300→5%Tween-80→45% saline Solubility: ≥ 2.5mg/mL (5.21mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (5.21mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 EtOH 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
	3.请依序添加每种溶剂：10% EtOH→90% (20% SBE-β-CD in saline Solubility: ≥ 2.5mg/mL (5.21mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (5.21mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 EtOH 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。
	4.请依序添加每种溶剂：10% EtOH→90% corn oil Solubility: ≥ 2.5mg/mL (5.21mM); Clear solution
	此⽅案可获得 ≥ 2.5mg/mL (5.21mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 EtOH 储备液加到 900μL ⽟⽶油中，混合均匀。
	5.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.08mg/mL (4.34mM); Clear solution
	此⽅案可获得 ≥ 2.08mg/mL (4.34mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。
	6.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.08mg/mL (4.34mM); Clear solution
	此⽅案可获得 ≥ 2.08mg/mL (4.34mM，饱和度未知) 的澄清溶液。以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。
	7.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.08mg/mL (4.34mM); Clear solution
	此⽅案可获得 ≥ 2.08mg/mL (4.34mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以 1mL ⼯作液为例，取 100μL 20.8mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。
＜1mg/ml表示微溶或不溶。
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

制备储备液

浓度溶液体积质量	1mg	5mg	10mg
1mM	2.0850mL	10.4251mL	20.8503mL
5mM	0.4170mL	2.0850mL	4.1701mL
10mM	0.2085mL	1.0425mL	2.0850mL

生物活性

产品描述	细胞松弛素B是真菌Drechslera dematoidea的代谢物，其结合肌动蛋白丝的倒刺端，导致亚基的缔合和解离两者的抑制。可用于肌动蛋白聚合研究和细胞学研究。
靶点	Kd: 2.2nM (F-actin, withmg2+), 1.4nM (F-actin, withmg2+/K+ )
体外研究	Cytochalasin B is a cell-permeable mycotoxin binding to the barbed end of actin filaments, inhibits the enlongation and shortening of actin filaments, with Kds of 2.2nM and 1.4nM for F-actin in the presence ofmgCl2 (2mM) ormgCl2 (2mM) plus KCl, respectively. Cytochalasin B (0.1-10μM) shows inhibitory effect on multiple murine cancer cell lines, with IC50s of 2.56μM (M109c), 10.46μM (B16BL6), 105.5μM (P388/ADR), 51.9μM (P388/S) and IC80s of 12.23μM (M109c), 44.86μM (B16BL6), 188.4μM (P388/ADR), 84.1μM (P388/S) after treatment for 3 h, with IC50s of 0.25μM (M109c), 0.37μM (B16F10), 0.87μM (B16BL6), and IC80s of 0.75μM (M109c), 1.21μM (B16F10), 10.41μM (B16BL6) after treatment for 4 days. Cytochalasin B (6μM) increases the myofibrillar fragmentation index (MFI), which is attributed to the intensely breaking of myofibrillar proteins into short segments. Cytochalasin B also accelerates the disruption of actin filaments. In addition, Cytochalasin B accelerates the transformation from F-actin to G-actin, lowering the content of F-actin and significantly increasing G-actin bands during postmortem conditioning
体内研究	Cytochalasin B (10, 25, 50mg/kg, i.p.) dose-dependently increases the life expectancy of Balb/c mice bearing with P388/ADR leukemias. Cytochalasin B at 50mg/kg produces 10 % long-term survival in the multidrug resistant P388/ADR cohort, and 40 % long-term survival in the drug sensitive P388/S cohort

推荐实验方法(仅供参考)

Cell Assay

The attached cell lines M109c, B16BL6, and B16F10 are seeded at 1 to 4 × 10⁴ cells/mL in 2mL volumes in 24-well culture plates 1 day prior to treatment with Cytochalasin B. The suspension culture of P388/ADR cells is seeded at 5 × 10⁴ cells/mL and allowed to grow overnight before Cytochalasin B treatment. Cells are treated with Cytochalasin B for 3 h, as well as 2, 3, or 4 days. In the case of continuous exposure for 2, 3, or 4 days, attached cells are trypsinized and counted with a hemacytometer. Leukemia cell suspensions are counted with a Coulter Counter. In the case of short-term exposure, cells are washed twice with fresh medium, then trypsinized (except for P388/ADR cells), reseeded, and allowed to regrow for 3 days, at which time they are counted. Growth results are calculated as the number of cells generated above the seeding density compared to the untreated control cells and graphically presented as percent of control increase

Animal Administration

Mice

For chemotherapy testing, Balb/c mice under isoflurane anesthesia are challenged with 2 × 10⁵ trypan blue negative P388/S or P388/ADR cells subcutaneously (s.c.) in a volume of 200μL. Untreated mice are kept in order to determine the lethality of the challenge without chemotherapeutic intervention. Long-term survival is defined as challenged mice that survive the duration of the observation period. Cytochalasins B and D are prepared in suspension form in 2 % carboxymethyl cellulose 1 % tween 20 (CMC/Tw) for intraperitoneal (i.p.) administration. The congeners or the vehicle are administered to leukemiachallenged mice on Days 1-8 following the initial challenge

保存条件：

-20℃

UN码：

HazardClass：

危害声明：

安全说明：

搜索质检报告(COA)

参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

质量 =

浓度 *

体积 *

分子量

*选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入输出 )

* = *

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

摩尔浓度计算公式

稀释公式

连续稀释计算器方程

连续稀释

计算结果