| 中文名称: | CTX1 | ||||
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| 英文名称: | CTX1 | ||||
| 别名: | 3,6-二氨基-9-吖啶甲腈 3,6-Diamino-9-acridinecarbonitrile | ||||
| CAS No: | 501935-96-2 | 分子式: | C14H10N4 | 分子量: | 234.26 |
| CAS No: | 501935-96-2 | ||||
| 分子式: | C14H10N4 | ||||
| 分子量: | 234.26 | ||||
基本信息
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产品编号：C10863 |
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产品名称：CTX1 |
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CAS: |
501935-96-2 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
234.26 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
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Ethanol |
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Water |
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体内（现配现用） |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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生物活性
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产品描述 |
一种 p53 激活物，可以克服 HdmX 介导的 p53 抑制。 |
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靶点/IC50 |
p53 |
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体外研究 |
CTX1 binds directly to HdmX to prevent p53-HdmX complex formation, resulting in the rapidly induction of p53 in a DNA damage-independent manner. |
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体内研究 |
CTX1 ( (30mg/kg; i.p.; five days a week; for 3 weeks) significantly enhances the survival of mice in AML model system.CTX1 exhibited significant anti-cancer activity alone as well as in combination with nutlin-3. |
推荐实验方法(仅供参考)
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激酶实验： |
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Exponentially growing OCI cells cultures are treated with 3μM CTX1, 8 μM Nutlin and or 15μM RO-5963 for 4.5 hrs. Wholecell extracts are generated using modified RIPA lysis buffer 25mM Tris (pH 8.0), 100mM NaCl, 0.5mM EDTA, 0.50% NP-40 and complete protease mixture tablet. Protein extracts (~750μg) are precleared and immune precipitation is performed using a kit according to the manufacturer's protocol. For the immunoprecipitation, mouse monoclonal anti-p53 and rabbit polyclonal anti-HDMX are used. Immune complexes are then collected, proteins are eluted and subjected to Western blotting with the indicated antibodies. |
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动物实验: |
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6 week old female NOD/SCID/IL2Rγ−/− mice are injected into the tail vein with 5×106 cells primary human AML cells (n=5 per group). Drug treatment is started 2 days after tumor cell injection. Nutlin-3 is given by oral gavage (200mg/kg) and CTX1 is injected i.p. (30mg/kg) five days a week for 3 weeks. Flow cytometry is performed on bone marrow cells isolated from the mouse femur using a human specific CD45PE antibody using a cytometer to confirm AML infiltration into the bone marrow. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
