基本信息
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产品编号： |
C10833 |
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产品名称： |
C2 Ceramide |
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CAS: |
3102-57-6 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
341.53 |
-20℃ |
一个月 |
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化学名： |
N-Acetyl-D-sphingosine |
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Solubility (25°C)： |
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体外:
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DMSO |
20mg/mL(58.56mM;Need ultrasonic and warming) |
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Ethanol |
17mg/mL(49.78mM;Need ultrasonic and warming) |
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Water |
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体内(现配现用): |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
2.9280mL |
14.6400mL |
29.2800mL |
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5mM |
0.5856mL |
2.9280mL |
5.8560mL |
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10mM |
0.2928mL |
1.4640mL |
2.9280mL |
生物活性
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产品描述 |
一种角质层的主要脂质，也是一种蛋白质磷酸酶 1 (PP1) 的激活剂。 |
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靶点 |
Protein phosphatase 1 PP2A Ceramide-activated protein phosphatase (CAPP) Apoptosis Mitochondrial respiratory chain complex III |
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体外研究 |
C2 Ceramide (5nM-200µM; 24 hours; primary mouse osteoblasts) treatment (≤500nM) promots osteoblast viability, whilst concentrations ≥2µM significantly reduces osteoblast viability in a dose- and time-dependent manner C2 Ceramide increases cytoplasmic histone-associated DNA fragments by 5.7- and 11.2-fold at 50µM and 100µM C2 Ceramide concentrations respectively in osteoblasts. At these higher concentrations, C2 Ceramide is a potent inducer of apoptosis in osteoblasts C2 Ceramide up-regulates mRNA expression of angiogenic genes in human dental pulp cells (HDPCs) and increases the migration and capillary tube formation of endothelial cells, whereas PP1 small interfering RNA shows opposite effects. Human dental pulp cells (HDPCs) increases levels of bone morphogenetic protein 2, phosphorylation of Smad 1/5/8, and mRNA expression of runt-related transcription factor 2 and osterix Cell Viability Assay |
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Cell Line: |
Primary mouse osteoblasts |
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Concentration: |
5nM-200µM |
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Incubation Time: |
24 hours |
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Result: |
Murine osteoblasts demonstrated a dose-dependent increase in their survival rate when exposed to low concentrations of 5-500 n M. Increasing concentrations of 20-200µM caused a dose-dependent decrease in mitochondrial succinate dehydrogenase activity and osteoblast survival. |
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体内研究 |
The PP1 activator C2 Ceramide increases alkaline phosphatase activity, mineralizes nodule formation, and mRNA expression of dentin matrix protein 1 and dentin sialophosphoprotein. In contrast, knockdown by PP1 small interfering RNA inhibits odontoblastic differentiation |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
