| 中文名称: | CPTH2 | ||||
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| 英文名称: | CPTH2 | ||||
| 别名: | CPTH2 国华试剂 CPTH2;HISTONE ACETYLTRANSFERASE INHIBITOR IV, CPTH2;Cyclopentylidene-[4-(4-chlorophenyl)thiazol-2-yl)hydrazone | ||||
| CAS No: | 357649-93-5 | 分子式: | C14H14ClN3S | 分子量: | 291.8 |
| CAS No: | 357649-93-5 | ||||
| 分子式: | C14H14ClN3S | ||||
| 分子量: | 291.8 | ||||
基本信息
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产品编号：C10820 |
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产品名称：CPTH2 |
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CAS: |
357649-93-5 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃(避光) |
六个月 |
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分子量： |
291.80 |
-20℃(避光) |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
58mg/mL (198.76mM) |
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Ethanol |
Insoluble |
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Water |
Insoluble |
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体内（现配现用） |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.4270mL |
17.1350mL |
34.2700mL |
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5mM |
0.6854mL |
3.4270mL |
6.8540mL |
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10mM |
0.3427mL |
1.7135mL |
3.4270mL |
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50mM |
0.0685mL |
0.3427mL |
0.6854mL |
生物活性
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产品描述 |
一种有效的组蛋白乙酰基转移酶 (HAT) 抑制剂。CPTH2 选择性地抑制 Gcn5 对组蛋白 H3 的乙酰化。 |
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靶点/IC50 |
HAT |
Gcn5p |
KAT3B |
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体外研究 |
CPTH2 (100μM;12,24,48 hours) causes a decrease in cell proliferation after as early as 12h with a further significant reduction after 48h stimulation.CPTH2 (100μM;12 or 48 hours) causes a comparable drop of the activity in both cell lines.CPTH2 (100μM; 48 hours) produces a drastic increase in apoptotic/dead cell population after 48h.CPTH2 (100μM;12,24,48 hours) shows a reduced acetylation of both global AcH3 histone and H3AcK18.CPTH2 (100μM;24,48 hours) is capable to counteract invasion and migration of ccRCC-786-O cells in culture.CPTH2 (0.2,0.5,1mM) inhibits the growth of a GCN5 deleted strain and a single catalytic mutant E173H.CPTH2 (0.6,0.8mM;for 24 hours) inhibits histone H3 acetylation in yeast cell cultures.CPTH2 inhibits the Gcn5p dependent functional network. Cell Proliferation Assay |
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Cell Line: |
Papillary thyroid (K1) and clear cell Renal Cell Carcinoma (ccRCC-786-O) cell lines |
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Concentration: |
100μM |
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Incubation Time: |
12,24,48 hours |
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Result: |
Caused a decrease in cell proliferation after as early as 12h with a further significant reduction after 48h stimulation. |
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Cell Viability Assay |
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Cell Line: |
K1 and ccRCC-786-O cell lines |
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Concentration: |
100μM |
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Incubation Time: |
24 hours (K1 cell) and 48 hours (ccRCC-786-O cell) |
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Result: |
Caused a comparable drop of the activity in both cell lines. |
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Apoptosis Analysis |
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Cell Line: |
ccRCC-786-O cells |
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Concentration: |
100μM |
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Incubation Time: |
48 hours |
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Result: |
Produced a drastic increase in apoptotic/dead cell population after 48h. |
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Western Blot Analysis |
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Cell Line: |
ccRCC-786-O cells |
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Concentration: |
100μM |
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Incubation Time: |
12,24,48 hours |
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Result: |
Showed a reduced acetylation of both global AcH3 histone and H3AcK18. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
