基本信息
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产品编号： |
C10148 |
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产品名称： |
Capsaicin |
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CAS: |
404-86-4 |
储存条件 |
粉末 |
2-8℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
两年 |
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分子量 |
305.41 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C)： |
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体外:
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DMSO |
61mg/mL (199.73mM) |
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Ethanol |
61mg/mL (199.73mM) |
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Water |
Insoluble |
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体内(现配现用): |
1.请依序添加每种溶剂：10% EtOH→90% corn oil Solubility: ≥ 20mg/mL (65.49mM); Clear solution |
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此⽅案可获得 ≥ 20mg/mL (65.49mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取 100μL 200.0mg/mL 的澄清 EtOH 储备液加到 900μL ⽟⽶油中，混合均匀。 |
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2.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.5mg/mL (8.19mM); Clear solution |
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此⽅案可获得 ≥ 2.5mg/mL (8.19mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中，混合均匀；向上述体系中加⼊ 50μL Tween-80，混合均匀；然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 |
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3.请依序添加每种溶剂：10% DMSO→90% (20% SBE-β-CD in saline) Solubility: ≥ 2.5mg/mL (8.19mM); Clear solution |
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此⽅案可获得 ≥ 2.5mg/mL (8.19mM，饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL 20% 的 SBE-β-CD ⽣理盐⽔⽔溶液中，混合均匀。 |
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4.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility: ≥ 2.5mg/mL (8.19mM); Clear solution |
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此⽅案可获得 ≥ 2.5mg/mL (8.19mM，饱和度未知) 的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。 以 1mL ⼯作液为例，取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 900μL ⽟⽶油中，混合均匀。 |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.2743mL |
16.3714mL |
32.7429mL |
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5mM |
0.6549mL |
3.2743mL |
6.5486mL |
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10mM |
0.3274mL |
1.6371mL |
3.2743mL |
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50mM |
0.0655mL |
0.3274mL |
0.6549mL |
生物活性
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产品描述 |
Capsaicin ((E)-Capsaicin) 是辣椒中的活性成分，是⼀种 TRPV1 激动剂。Capsaicin 具有减轻疼痛、抗氧化、抗炎、神经 保护和抗癌作⽤。 |
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靶点 |
EC50: 290 nM (hTRPV1, in HEK293 cell) |
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体外研究 |
Capsaicin (50-300µM; 24-72 hours) shows an augmented decrease in cell growth in a dose- and time-dependent manner. The observed IC50 value is around 150µM. Capsaicin (50-300µM; 24-72 hours) shows increase in cytosolic cytochrome c, activation of caspase 3 and PARP (p85) levels, and decreases anti-apoptotic Bcl-2 protein and increases pro-apoptotic Bad/Bax expression. Capsaicin increases the nuclear condensation, nuclear DNA fragmentation and sub-G1 DNA content. Capsaicin suppresses the cell cycle progression at the G1/S phase in FaDu cells by decreasing the expression of the regulators of cyclin B1 and D1, as well as cyclin-dependent protein kinases cdk-1, cdk-2 and cdk-4 Cell Viability Assay |
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Cell Line: |
Human pharyngeal squamous carcinoma cells (FaDu) cells |
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Concentration: |
50µM, 100µM, 200µM, and 300µM |
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Incubation Time: |
24 hours, 48 hours and 72 hours |
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Result: |
Showed an augmented decrease in cell growth. |
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Apoptosis Analysis |
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Cell Line: |
FaDu cells |
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Concentration: |
50µM, 100µM and 200µM |
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Incubation Time: |
12 hours |
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Result: |
Increased the activity of caspase 3 in a time-dependent manner. |
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Western Blot Analysis |
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Cell Line: |
FaDu cells |
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Concentration: |
200µM |
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Incubation Time: |
24 hours |
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Result: |
The observed activation of caspase 3 and PARP (p85) levels. |
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体内研究 |
Capsaicin suppresses the development of lung carcinoma by amending the protein expressions of apoptotic regulators p53, Bcl-2, Bax and caspase-3 |
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Animal Model: |
Swiss albino mice (20-25 g; 8-10 weeks old) induced by Benzo(a)pyrene |
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Dosage: |
10mg/kg |
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Administration: |
Intraperitoneal administration; once in a week; for 14 weeks |
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Result: |
Inhibited the development of mice lung carcinogenesis. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
