中文名称: | BAM15 促销 | ||||
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英文名称: | BAM15 | ||||
别名: | N5,N6-双(2-氟苯基)-[1,2,5]恶二唑并[3,4-b]吡嗪-5,6-二胺 5-N,6-N-Bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine | ||||
CAS No: | 210302-17-3 | 分子式: | C16H10F2N6O | 分子量: | 340.29 |
CAS No: | 210302-17-3 | ||||
分子式: | C16H10F2N6O | ||||
分子量: | 340.29 |
基本信息
产品编号: |
B11358 |
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产品名称: |
BAM15 |
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CAS: |
210302-17-3 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
两年 |
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分子量 |
340.29 |
-20℃ |
一个月 |
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化学名: |
5-N,6-N-Bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine |
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Solubility (25°C): |
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体外:
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DMSO |
68mg/mL (199.82mM) |
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Ethanol |
Insoluble |
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Water |
Insoluble |
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体内(现配现用): |
1.请依序添加每种溶剂:10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility: ≥ 2.5mg/mL (7.35mM); Clear solution |
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此⽅案可获得 ≥ 2.5mg/mL (7.35mM,饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例,取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中,混合均匀;向上述体系中加⼊ 50μL Tween-80,混合均匀;然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL。 |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
2.9387mL |
14.6933mL |
29.3867mL |
5mM |
0.5877mL |
2.9387mL |
5.8773mL |
10mM |
0.2939mL |
1.4693mL |
2.9387mL |
50mM |
0.0588mL |
0.2939mL |
0.5877mL |
生物活性
产品描述 |
线粒体质子解偶联剂。BAM15是氧化磷酸化(OXPHOS)解偶联剂。 |
靶点 |
AMPK |
体外研究 |
BAM 15 is able to increase O2 consumption across a broad dosing range without increasing ROS. BAM 15 and FCCP are structurally unrelated and it is observed that low doses of BAM 15 from 100 nM to 1μM increase cellular O2 consumption rate (OCR) to a similar degree as FCCP, but higher concentrations from 1μM to 50μM reveal that BAM 15 is able to maintain uncoupled respiration at a high rate in a range of cell lines. BAM 15 is fully capable of increasing mitochondrial respiration in the presence of oligomycin and does so across a broader concentration range than FCCP in both myoblasts and hepatocytes. BAM 15 induces mitochondrial swelling, demonstrating that BAM 15 is a protonophore. BAM15-treated cells are more viable than FCCP-treated cells when administered across a broad dosing range up to 50μM |
体内研究 |
Compare to vehicle-treated mice, animals that receive BAM 15 are protected from kidney injury as indicated by lower plasma creatinine levels at 24 and 48 h post-ischemia, reduced tubular necrosis, less depletion of brush border villi, less obstruction of proximal tubules, and less immune cell infiltration |
推荐实验方法(仅供参考)
Kinase Assay |
Electron flow assays are performed. Briefly, 5 μg of mitochondrial protein in MAS is loaded into a 24-well tissue culture plate and centrifuged at 2000×g for 15 min at 4°C. Prior to the assay, mitochondria are incubated at 37°C for 10 mins in MAS containing 10mM pyruvate, 2mM malate, and 5μM BAM 15 or FCCP. Rotenone (2μM), succinate (10mM), antimycin A (4μM), and N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD, 100μM) plus ascorbate (10mM) are added sequentially as indicated in the figure. N=3 wells/plate of a representative of 3 plates |
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Cell Assay |
L6 cells are incubated with the fluorescent indicator of mitochondrial membrane potential tetramethylrhodamine (TMRM, 125 nM) or DMSO (1%) control for 30 min. The cells are then centrifuged for 5 min at 700×g and resuspended in unbuffered DMEM at a concentration of 1×105 cells/mL. The cells are then treated with BAM 15 or DMSO (0.1%) for 10 min prior to flow cytometric analysis |
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Animal Administration |
Male mice (8-week old, C57BL/6) are used. Mice are i.p. injected with BAM 15 at 1 or 5mg/kg, 1 h before kidney IR. Vehicle mice are also injected with the same solution BAM 15 is prepared with (3% DMSO in 50% PEG400) |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )