中文名称: BAM15 促销
英文名称: BAM15
CAS No: 210302-17-3
分子式: C16H10F2N6O
分子量: 340.29
B11358 BAM15 ≥98% (psaitong)
包装规格:
5mg 10mg 25mg 100mg in glass bottle
溶解性:
溶于DMSO(20mg/ml)
产品描述:

基本信息

产品编号:

B11358

产品名称:

BAM15

CAS:

210302-17-3

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C16H10F2N6O

溶于液体

-80℃

两年

分子量

340.29

-20℃

一个月

化学名: 

5-N,6-N-Bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine

Solubility (25°C):

 

体外:

 

DMSO

68mg/mL (199.82mM)

Ethanol

Insoluble

Water

Insoluble

体内(现配现用):

1.请依序添加每种溶剂:10% DMSO40% PEG3005% Tween-8045% saline

Solubility: ≥ 2.5mg/mL (7.35mM); Clear solution

此⽅案可获得 ≥ 2.5mg/mL (7.35mM,饱和度未知) 的澄清溶液。 以 1mL ⼯作液为例,取 100μL 25.0mg/mL 的澄清 DMSO 储备液加到 400μL PEG300 中,混合均匀;向上述体系中加⼊ 50μL Tween-80,混合均匀;然后继续加⼊ 450μL ⽣理盐⽔定容⾄ 1mL

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

2.9387mL

14.6933mL

29.3867mL

5mM

0.5877mL

2.9387mL

5.8773mL

10mM

0.2939mL

1.4693mL

2.9387mL

50mM

0.0588mL

0.2939mL

0.5877mL

 

生物活性

产品描述

线粒体质子解偶联剂。BAM15是氧化磷酸化(OXPHOS)解偶联剂。

靶点

AMPK 
()

体外研究

BAM 15 is able to increase O2 consumption across a broad dosing range without increasing ROS. BAM 15 and FCCP are structurally unrelated and it is observed that low doses of BAM 15 from 100 nM to 1μM increase cellular O2 consumption rate (OCR) to a similar degree as FCCP, but higher concentrations from 1μM to 50μM reveal that BAM 15 is able to maintain uncoupled respiration at a high rate in a range of cell lines. BAM 15 is fully capable of increasing mitochondrial respiration in the presence of oligomycin and does so across a broader concentration range than FCCP in both myoblasts and hepatocytes. BAM 15 induces mitochondrial swelling, demonstrating that BAM 15 is a protonophore. BAM15-treated cells are more viable than FCCP-treated cells when administered across a broad dosing range up to 50μM

体内研究

Compare to vehicle-treated mice, animals that receive BAM 15 are protected from kidney injury as indicated by lower plasma creatinine levels at 24 and 48 h post-ischemia, reduced tubular necrosis, less depletion of brush border villi, less obstruction of proximal tubules, and less immune cell infiltration

 

推荐实验方法(仅供参考)

Kinase Assay

Electron flow assays are performed. Briefly, 5 μg of mitochondrial protein in MAS is loaded into a 24-well tissue culture plate and centrifuged at 2000×g for 15 min at 4°C. Prior to the assay, mitochondria are incubated at 37°C for 10 mins in MAS containing 10mM pyruvate, 2mM malate, and 5μM BAM 15 or FCCP. Rotenone (2μM), succinate (10mM), antimycin A (4μM), and N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD, 100μM) plus ascorbate (10mM) are added sequentially as indicated in the figure. N=3 wells/plate of a representative of 3 plates

 

 

Cell Assay

L6 cells are incubated with the fluorescent indicator of mitochondrial membrane potential tetramethylrhodamine (TMRM, 125 nM) or DMSO (1%) control for 30 min. The cells are then centrifuged for 5 min at 700×g and resuspended in unbuffered DMEM at a concentration of 1×105 cells/mL. The cells are then treated with BAM 15 or DMSO (0.1%) for 10 min prior to flow cytometric analysis

 

 

Animal Administration

Male mice (8-week old, C57BL/6) are used. Mice are i.p. injected with BAM 15 at 1 or 5mg/kg, 1 h before kidney IR. Vehicle mice are also injected with the same solution BAM 15 is prepared with (3% DMSO in 50% PEG400)

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
搜索质检报告(COA)
参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):