| 中文名称: | BTB-1 | ||||
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| 英文名称: | BTB-1 | ||||
| 别名: | 4-氯-2-硝基-1-(苯基磺酰基)苯 4-Chloro-2-nitro-1-(phenylsulfonyl)benzene | ||||
| CAS No: | 86030-08-2 | 分子式: | C12H8ClNO4S | 分子量: | 297.71 |
| CAS No: | 86030-08-2 | ||||
| 分子式: | C12H8ClNO4S | ||||
| 分子量: | 297.71 | ||||
| EINEC: | 156750 | ||||
| EINEC: | 156750 | ||||
基本信息
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产品编号：B10789 |
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产品名称：BTB-1 |
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CAS: |
86030-08-2 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
六个月 |
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分子量 |
297.71 |
-20℃ |
一个月 |
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化学名： |
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Solubility (25°C) |
体外 |
DMSO |
60mg/mL (201.53mM) |
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Ethanol |
2mg/mL (6.71mM) |
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Water |
Insoluble |
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体内（现配现用） |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.3590mL |
16.7949mL |
33.5897mL |
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5mM |
0.6718mL |
3.3590mL |
6.7179mL |
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10mM |
0.3359mL |
1.6795mL |
3.3590mL |
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50mM |
0.0672mL |
0.3359mL |
0.6718mL |
生物活性
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产品描述 |
一种有效，选择性和可逆的有丝分裂运动蛋白Kif18A的抑制剂，IC50值为1.69μM。 |
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靶点/IC50 |
Kif18A |
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1.69μM |
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体外研究 |
BTB-1 blocks the motility of Kif18A in a reversible manner. BTB-1 inhibits Kif18A in an adenosine triphosphate (ATP)-competitive but microtubule-uncompetitive manner and slows down the progression of cells through mitosis. 100μM BTB-1 does not significantly inhibit any of the other tested mitotic kinesins. BTB-1 competes with ATP for Kif18A binding only when the motor-protein is associated with its pseudosubstrate microtubules. HeLa cells treated with BTB-1 accumulate in mitosis in a dose-dependent manner. BTB-1 shows cell toxicity with an EC50 values of 35.8μM. HeLa cells treated with 50μM BTB1 reveals severe defects in spindle morphology and chromosome alignment. Treatment with high concentrations of BTB-1 does not result in elongated spindles. |
推荐实验方法(仅供参考)
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激酶实验： |
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BTB-1 is prepared in DMSO. The activity of His-Kif18Amotor at increasing concentrations of ATP is monitored in the presence of 3μM Mts and increasing concentrations of BTB-1 (0.21μM,0.42μM,0.85μM,1.7μM) or DMSO as control. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
