中文名称: (E/Z)-BCI
英文名称: (E/Z)-BCI
CAS No: 15982-84-0
分子式: C22H23NO
分子量: 317.42
EINEC: 150117
B10750 (E/Z)-BCI ≥95% (psaitong)
包装规格:
10mg in glass bottle
产品描述:

基本信息

产品编号:

B10750

产品名称:

(E/Z)-BCI

CAS:

15982-84-0

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C22H23NO

溶于液体

-80℃

两年

分子量

317.42

 

 

化学名: 

(E)-2-Benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one

Solubility (25°C):

 

体外:

 

DMSO

63mg/mL(198.47mM)

Ethanol

20mg/mL(63.0mM)

Water

Insoluble

体内(现配现用):

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

3.1504mL

15.7520mL

31.5040mL

5mM

0.6301mL

3.1504mL

6.3008mL

10mM

0.3150mL

1.5752mL

3.1504mL

50mM

0.0630mL

0.3150mL

0.6301mL

 

生物活性

产品描述

一种DUSP6抑制剂,具有抗炎活性。(E/Z)-BCI通过激活Nrf2信号和抑制NF-κB通路,减弱LPS诱导的巨噬细胞炎症和ROS生成。

靶点

ROS 
()

DUSP1 
(Cell-free assay)

8.0μM(EC50)

DUSP6 
(Cell-free assay)

13.3μM(EC50)

体外研究

(E/Z)-BCI hydrochloride (2-10μM; 72 hours) significantly decreases cell viability in a time and dose-dependent manner in gastric epithelial cell GES1, GC cell lines, and AGS cell lines.

(E/Z)-BCI hydrochloride (0.5-4μM; 24 hours) significantly inhibits DUSP6 expression in LPS-activated macrophages. (E/Z)-BCI hydrochloride (0.5-2μM; 24 hours) treatment significantly inhibits the expression of IL-1β, TNF-α and IL-6 mRNA in LPS-activated macrophages.

(E/Z)-BCI hydrochloride decreases ROS production and activates the Nrf2 pathway in LPS-activated macrophages.

(E/Z)- BCI hydrochloride inhibits cell proliferation, migration and invasion in a receptor-independent manner and enhances Cisplatin (CDDP) cytotoxicity (enhances CDDP-induced cell death and apoptosis) at pharmacological concentrations in the gastric cancer (GC) cells

Cell Viability Assay

Cell Line:

Gastric epithelial cell GES1, GC cell lines (HGC27, SGC7901, MKN45, BGC823,mgC803, SNU216, NUGC4), AGS cell lines

Concentration:

2μM,4μM,6μM,8μM,10μM

Incubation Time:

72 hours

Result:

Cell viability was significantly decreased in a time and dose-dependent manner.

Western Blot Analysis

Cell Line:

RAW264.7 macrophage cells (by LPS-activated macrophages)

Concentration:

0.5μM,1μM,2μM,4μM

Incubation Time:

24 hours

Result:

DUSP6 protein was significantly downregulated in LPS-activated macrophages.

RT-PCR

Cell Line:

RAW264.7 macrophage cells (by LPS-activated macrophages)

Concentration:

0.5μM,1μM,2μM

Incubation Time:

24 hours

Result:

The expression of IL-1β, TNF-α and IL-6 mRNA was significantly inhibited in LPS-activated macrophages.

体内研究

(E/Z)-BCI hydrochloride (35mg/kg; intraperitoneal injection; every 7 days; for four weeks; female BALB/c nude mice) treatment enhances cisplatin efficacy in PDX models

Animal Model:

Patient-derived xenograft (PDX) models (4-5-week-old female BALB/c nude mice)

Dosage:

35mg/kg

Administration:

Intraperitoneal injection; every 7 days; for four weeks

Result:

Tumor weights in the PDX models treated plus CDDP were significantly suppressed compared with tumors from PDX model mice treated with either agent alone.

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
搜索质检报告(COA)
参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):