中文名称: | (E/Z)-BCI | ||||
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英文名称: | (E/Z)-BCI | ||||
别名: | NSC 150117;(E)-2-Benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one | ||||
CAS No: | 15982-84-0 | 分子式: | C22H23NO | 分子量: | 317.42 |
CAS No: | 15982-84-0 | ||||
分子式: | C22H23NO | ||||
分子量: | 317.42 | ||||
EINEC: | 150117 | ||||
EINEC: | 150117 |
基本信息
产品编号: |
B10750 |
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产品名称: |
(E/Z)-BCI |
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CAS: |
15982-84-0 |
储存条件 |
粉末 |
-20℃ |
四年 |
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分子式: |
溶于液体 |
-80℃ |
两年 |
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分子量 |
317.42 |
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化学名: |
(E)-2-Benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one |
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Solubility (25°C): |
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体外:
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DMSO |
63mg/mL(198.47mM) |
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Ethanol |
20mg/mL(63.0mM) |
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Water |
Insoluble |
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体内(现配现用): |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
3.1504mL |
15.7520mL |
31.5040mL |
5mM |
0.6301mL |
3.1504mL |
6.3008mL |
10mM |
0.3150mL |
1.5752mL |
3.1504mL |
50mM |
0.0630mL |
0.3150mL |
0.6301mL |
生物活性
产品描述 |
一种DUSP6抑制剂,具有抗炎活性。(E/Z)-BCI通过激活Nrf2信号和抑制NF-κB通路,减弱LPS诱导的巨噬细胞炎症和ROS生成。 |
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靶点 |
ROS |
DUSP1 8.0μM(EC50) |
DUSP6 13.3μM(EC50) |
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体外研究 |
(E/Z)-BCI hydrochloride (2-10μM; 72 hours) significantly decreases cell viability in a time and dose-dependent manner in gastric epithelial cell GES1, GC cell lines, and AGS cell lines. (E/Z)-BCI hydrochloride (0.5-4μM; 24 hours) significantly inhibits DUSP6 expression in LPS-activated macrophages. (E/Z)-BCI hydrochloride (0.5-2μM; 24 hours) treatment significantly inhibits the expression of IL-1β, TNF-α and IL-6 mRNA in LPS-activated macrophages. (E/Z)-BCI hydrochloride decreases ROS production and activates the Nrf2 pathway in LPS-activated macrophages. (E/Z)- BCI hydrochloride inhibits cell proliferation, migration and invasion in a receptor-independent manner and enhances Cisplatin (CDDP) cytotoxicity (enhances CDDP-induced cell death and apoptosis) at pharmacological concentrations in the gastric cancer (GC) cells Cell Viability Assay |
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Cell Line: |
Gastric epithelial cell GES1, GC cell lines (HGC27, SGC7901, MKN45, BGC823,mgC803, SNU216, NUGC4), AGS cell lines |
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Concentration: |
2μM,4μM,6μM,8μM,10μM |
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Incubation Time: |
72 hours |
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Result: |
Cell viability was significantly decreased in a time and dose-dependent manner. |
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Western Blot Analysis |
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Cell Line: |
RAW264.7 macrophage cells (by LPS-activated macrophages) |
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Concentration: |
0.5μM,1μM,2μM,4μM |
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Incubation Time: |
24 hours |
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Result: |
DUSP6 protein was significantly downregulated in LPS-activated macrophages. |
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RT-PCR |
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Cell Line: |
RAW264.7 macrophage cells (by LPS-activated macrophages) |
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Concentration: |
0.5μM,1μM,2μM |
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Incubation Time: |
24 hours |
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Result: |
The expression of IL-1β, TNF-α and IL-6 mRNA was significantly inhibited in LPS-activated macrophages. |
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体内研究 |
(E/Z)-BCI hydrochloride (35mg/kg; intraperitoneal injection; every 7 days; for four weeks; female BALB/c nude mice) treatment enhances cisplatin efficacy in PDX models |
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Animal Model: |
Patient-derived xenograft (PDX) models (4-5-week-old female BALB/c nude mice) |
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Dosage: |
35mg/kg |
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Administration: |
Intraperitoneal injection; every 7 days; for four weeks |
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Result: |
Tumor weights in the PDX models treated plus CDDP were significantly suppressed compared with tumors from PDX model mice treated with either agent alone. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )