中文名称: 3BDO 促销
英文名称: 3BDO
CAS No: 890405-51-3
分子式: C18H17NO5
分子量: 327.33
B10721 3BDO ≥98% (psaitong)
包装规格:
5mg 10mg 25mg 100mg in glass bottle
溶解性:
溶于DMSO(≥ 100 mg/mL)
产品描述:

基本信息

产品编号:

B10721

产品名称:

3BDO

CAS:

890405-51-3

 

储存条件

粉末

-20℃

四年

 

 

分子式:

C18H17NO5

溶于液体

-80℃

6个月

分子量:

327.33

-20℃

1个月

化学名: 

3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one

Solubility (25°C):

 

体外:

 

DMSO

65mg/mL (198.57mM)

Ethanol

59mg/mL (180.24mM)

Water

Insoluble

体内(现配现用):

请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照In Vitro⽅式配制澄清的储备液,再依次添加助溶剂:为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的⼯作液,建议您现⽤现配,当天使⽤;以下溶剂前显⽰的百分⽐是指该溶剂在您配制终溶液中的体积占⽐;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的⽅式助溶

1.请依序添加每种溶剂:10% DMSO40% PEG3005% Tween-8045% saline

Solubility:≥2.5mg/mL(7.64mM);Clear solution

2.请依序添加每种溶剂:10% DMSO90%(20% SBE-β-CD in saline)

Solubility:2.5mg/mL(7.64mM);Suspended solution;Need ultrasonic

3.请依序添加每种溶剂:10% DMSO90% corn oil

Solubility:≥2.5mg/mL(7.64mM);Clear solution

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

3.0550mL

15.2751mL

30.5502mL

5mM

0.6110mL

3.0550mL

6.1100mL

10mM

0.3055mL

1.5275mL

3.0550mL

 

生物活性

产品描述

一种新型mTOR激活剂,也能抑制自噬。

靶点

mTOR

体外研究

Phosphorylation of RPS6KB1 and EIF4EBP1 is significantly increased by 3BDO with vector alone but suppressed with FKBP1A overexpression.Rapamycin fails to decrease the phosphorylation of MTOR and RPS6KB1 in the presence of 3BDO.3BDO suppresses the increase in MAP1LC3B puncta induced with rapamycin.3BDO also inhibits the effect of rapamycin in HUVECs.The phosphorylation of Ser residues is decreased in HUVECs treated with 10μM rapamycin,and 60μM 3BDO reverses the phosphorylation.The results show that 3BDO suppresses the increased MAP1LC3B puncta number,MAP1LC3B-II level and decreased SQSTM1 protein level induced by rapamycin.3BDO could dose- and time-dependently decrease FLJ11812 level in HUVECs.Overexpression of FLJ11812 reverses the inhibition of autophagy induced by 3BDO.

体内研究

Immunofluorescence assay reveals that 3BDO treatment increases the level of p-p70S6K and decreases the protein level of ATG13 in plaque endothelium of mice.3BDO does not affect the phosphorylation of mTOR direct downstream targets p70S6K and 4EBP1.As compare with controls,apoE-/- mice show inhibited endothelium autophagy and apoptosis with 3BDO treatment,so 3BDO protects against endothelium injury in atherosclerosis.3BDO treatment stabilizes established atherosclerotic lesions in apoE-/-mice.In apoE-/-mice,as compare with controls,with 3BDO treatment,the serum level of IL6 and IL-8 is significantly decreased.

 

推荐实验方法(仅供参考)

激酶实验:

Total protein is obtained from HUVECs by using of IP lysis buffer after treatment with rapamycin (10μM),3BDO (60μM) or both for 6h.After centrifuging at 4℃,the supernatant is collected and incubated with protein A/G agarose beads and TIA1 antibody or normal mouse IgG as a control at 4℃ overnight.The beads are washed 3 times with IP lysis buffer and then eluted with 4×SDS loading buffer.Ser phosphorylation is detected by western blot assay with Ser phosphorylation antibody.

 

细胞实验:

 

HUVECs are isolated from umbilical cords and cultured in M199 medium with 20% (v/v) fetal bovine serum and 10 IU/mL fibroblast growth factor 2 (FGF2) in a humidified incubator at 37℃ with 5% CO2.Cells up to passage 10 are used for experiments.When HUVECs are grown to 80% confluency,HUVECs are treated with DMSO or 60µM 3BDO for 24h,then total RNA is extracted.

 

动物实验:

 

Male apoE-/- mice (8 weeks old) are used in this study.ApoE-/- mice are fed an atherogenic diet (containing 21% fat and 0.15% cholesterol).To avoid the potential confounding effects of variation among batches of diet,a single batch is reserved and used throughout the experiment.Mice at 20 weeks older are divided into 3 groups for treatment (n=8 mice/group) for 8 weeks:control (DMSO),low-dose 3BDO (50mg/kg/d;3BDO-L) and high-dose 3BDO (100mg/kg/d;3BDO-H).The body weight of mice is measured every week during 3BDO injection.Blood samples are taken from the inferior vena cava,and animals are killed by exsanguination.

 

保存条件:
-20℃
UN码:
HazardClass:
危害声明:
安全说明:
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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

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用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

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连续稀释计算器方程

  • 连续稀释

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  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):