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中文名称: BIO-acetoxime
英文名称: BIO-acetoxime
CAS No: 667463-85-6
分子式: C18H12BrN3O3
分子量: 398.21
安全技术说明书(PDF) MSDS(PDF) 点击下载产品说明书(PDF)
B10587 BIO-acetoxime ≥98% (psaitong)
包装规格：
5mg 10mg 25mg 50mg 100mg in glass bottle
溶解性：
溶于DMSO（20.83 mg/mL 超声)
产品描述：

基本信息

产品编号：

B10587

产品名称：

BIO-acetoxime

CAS:

667463-85-6

 

储存条件

粉末

-20℃

四年

 

 

分子式：

C18H12BrN3O3

溶于液体

-80℃

6个月

分子量：

398.21

-20℃

1个月

化学名： 

BIO-acetoxime (GSK-3 Inhibitor X)

Solubility (25°C)：

 

体外:

 

DMSO

39mg/mL warmed with 50ºC water bath (97.93mM)

Ethanol

Insoluble

Water

Insoluble

体内(现配现用):

请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照In Vitro⽅式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐是指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶

1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline

Solubility:≥2.08mg/mL(5.22mM);Clear solution

2.请依序添加每种溶剂：10% DMSO→90%(20% SBE-β-CD in saline)

Solubility:2.08mg/mL(5.22mM);Suspended solution;Need ultrasonic

3.请依序添加每种溶剂：10% DMSO→90% corn oil

Solubility:≥2.08mg/mL(5.22mM);Clear solution

＜1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

2.5112mL

12.5562mL

25.1124mL

5mM

0.5022mL

2.5112mL

5.0225mL

10mM

0.2511mL

1.2556mL

2.5112mL

 

生物活性

产品描述

一种有效、选择性的GSK-3抑制剂，对GSK-3α/β的IC50值均为10nM；BIO-acetoxime具有抗惊厥和抗感染作用。

靶点

GSK-3;Apoptosis;HSV

体外研究

BIO-acetoxime (BIA;Compound 13) is a potent and selective GSK-3α/β inhibitor,with IC50s of both 10nM.BIO-acetoxime (BIA) shows weak effect on CDK5/p35 (IC50,2.4μM),CDK2/cyclin A (IC50,4.3μM),and CDK1/cyclin B (IC50,63μM).BIOacetoxime (BIA) (5μM and 10μM) increases the viability of HSV-1-infected cells but shows little effect on morphology and viability of mockin-fected cells.Moreover,BIO-acetoxime (BIA) (0.625,1.25,2.5,5,and 10μM) significantly reduces the release of HSV-1 particles in OC3 cells,with an EC50 of 0.68±0.28μM.BIO-acetoxime (BIA) inhibits the expression of HSV-1 genes,and may not affect the nuclear targeting of HSV-1 capsids.In addition,delayed addition of BIO-acetoxime (BIA) also inhibits HSV-1 infection.

体内研究

BIO-acetoxime (BIA) shows anticonvulsant effects in the focal pilocarpine rat model at 0.5mg/kg,and in 6-Hz fully kindled FVB/N mice at 0.5,2.5,and 5 mg/kg via i.p.administration.

推荐实验方法(仅供参考)

细胞实验:

 

OC3 cells are seeded in 3.5- and 6-cm diameter dishes for western blot analysis and RNA isolation,respectively.Cells are mock treated or exposed to HSV-1 at a multiplicity of infection (m.o.i.) of 5 unless otherwise specified.The glycogen synthase kinase-3 (GSK-3) inhibitor BIO-acetoxime (BIA),is first dissolved in DMSO to make a stock solution.Medium containing DMSO serves as a solvent control.Cells are pretreated with medium only,medium containing 0.1% DMSO or BIO-acetoxime (BIA) for 45 min prior to mock or HSV-1 infection.BIO-acetoxime (BIA) is also present throughout the infection period.To examine the direct effect on viruses,HSV-1 is treated directly with medium containing 5μM BIOacetoxime (BIA) for 1h at room temperature.Cell morphology is observed with an inverted microscope.

 

动物实验:

 

Mice

Male FVB/N mice are used to test the anticonvulsant effects of BIO-acetoxime in fully kindled mice.To achieve the fully kindled state,mice are stimulated at a fixed subconvulsive threshold current twice daily,except the weekends,with a 4h minimum interval between stimulations.Kindled mice are then stimulated for only 2 days a week,twice daily to maintain the fully kindled state.Meanwhile nonkindled mice are kept at the original stimulation scheme for maximum 1-2 more weeks.The anticonvulsant effect of BIO-acetoxime (BIA) is investigated in the“epileptic”fully kindled mice by stimulating these animals on Monday morning.On Monday afternoon,the animals are intraperitoneally (i.p.) injected with vehicle,30 min prior to stimulation.On Tuesday morning,mice are again stimulated.On Tuesday afternoon,mice are injected i.p.with BIO-acetoxime (BIA) and are stimulated 30 min later.Seizure severity is assessed using Racine’s scale.The pretreatment scores are compared with the post-treatment scores,obtained after the treatment on Tuesday.To confirm any observed effects,the experiment is repeated,respecting 1 week of washout.During this washout week,all mice are stimulated for 2 days (twice daily) to maintain the kindled state.Compound testing during a longer period has not been validated so far.This means that only one dose of BIO-acetoxime can be tested in one batch of kindled mice.
保存条件：
-20℃
UN码：
HazardClass：
危害声明：
安全说明：
搜索质检报告(COA)
参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • =
    *
    *
    *选择对应的单位 *空出希望得到的变量，填写另外两个变量

用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
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