中文名称: 溴百里酚蓝 促销
英文名称: Bromothymol Blue
CAS No: 76-59-5
分子式: C27H28Br2O5S
分子量: 624.38
EINEC: 200-971-2
B10099 溴百里酚蓝 ACS (psaitong)
包装规格:
5g 25g 100g in glass bottle
溶解性:
熔点:200-202°C 溶于乙醇、乙醚、甲醇和稀氢氧化碱溶液,略溶于苯、甲苯和二甲苯,微溶于水,几乎不溶于石油醚,最大吸收波长420nm。
产品描述:

基本信息

产品编号:

B10099

产品名称:

Bromothymol Blue

CAS:

76-59-5

 

储存条件

粉末

室温

四年

分子式:

C27H28Br2O5S

溶于液体

-80℃

6个月

分子量:

624.38

-20℃

1个月

化学名: 

Bromothymol Blue,ACS reagent,Dye content 95%

Solubility (25°C):

 

体外:

 

DMSO

 

Ethanol

 

Water

 

体内(现配现用):

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

1.6016mL

8.0079mL

16.0159mL

5mM

0.3203mL

1.6016mL

3.2032mL

10mM

0.1602mL

0.8008mL

1.6016mL

 

生物活性

产品描述

酸碱指示剂,pH变色范围6.0(黄色)-7.6(蓝色)。吸附指示剂。

靶点

Others

体外研究

A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer.A pH-shift assay is developed on the basis of BTB as the pH indicator and potassium phosphate as the buffer component.Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate.The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5).Upon the addition of a Bromothymol Blue solution (0.1%,w/v) to an agar plate,zones surrounding colonies of K.vulgare 07 mutants change their color from blue to yellow because K.vulgare 07 mutants release 2-KLG on agar plates,thereby acidifying surrounding areas around colonies.

 

推荐实验方法(仅供参考)

激酶实验:

For all three enzymes,an expression in the 96-deep well scale is performed.Therefore,electrocompetent E.coli ArcticExpress(DE3) cells are transformed with the corresponding plasmid.Single clones are picked using the CP7200 Colony Picker and transferred to 96-deep well plates filled with 1.2mL autoinduction media by a MicroFlo Select dispenser.After incubation (36h,37℃ at 1,000 rpm),further processing is done manually. First,100μL of cell culture is transferred into a 96-well plate (U-shaped bottom) and harvested by centrifugation (4,570 rpm,10 min at RT) while the supernatant is discarded.The frozen pellets (1h at -20℃) are thawed at room temperature for one hour to improve cell lysis.Lysis is continued by the addition of 30μL lysis buffer (3h,1,000 rpm,37℃) containing 2mM KPi,pH 7.0,2mM MgCl2,10μg/mL DNaseI,100μg/mL lysozyme.Next,120μL buffer (2mM KPi,pH 7.0) is added followed by centrifugation (3,000 rpm,15 min at RT).For the photometric measurement,20μL of the crude extract is transferred to a 96-well plate (F-shaped bottom) and the reaction is started by adding 180μL master mix to give a final volume of 200μL (2.5mM KPi,pH 7.0,2mM MgCl2,25μg/mL BTB and 5mM keto-deoxy-D-glucarate).The measurements are carried out for 60 min at 2-min intervals.Depending on the enzyme,different time windows are used for the activity calculation.

保存条件:
室温
UN码:
HazardClass:
危害声明:
安全说明:
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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

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    *选择对应的单位 *空出希望得到的变量,填写另外两个变量

用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

  • 初始浓度:
  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):