中文名称: | 溴百里酚蓝 促销 | ||||
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英文名称: | Bromothymol Blue | ||||
别名: | 3,3″-二溴百里酚磺酞;溴百里香酚蓝 Bromothymol Blue;BTB | ||||
CAS No: | 76-59-5 | 分子式: | C27H28Br2O5S | 分子量: | 624.38 |
CAS No: | 76-59-5 | ||||
分子式: | C27H28Br2O5S | ||||
分子量: | 624.38 | ||||
MDL: | MFCD00005872 | EINEC: | 200-971-2 | ||
EINEC: | 200-971-2 |
基本信息
产品编号: |
B10099 |
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产品名称: |
Bromothymol Blue |
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CAS: |
76-59-5 |
储存条件 |
粉末 |
室温 |
四年 |
分子式: |
溶于液体 |
-80℃ |
6个月 |
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分子量: |
624.38 |
-20℃ |
1个月 |
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化学名: |
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Solubility (25°C): |
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体外:
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DMSO |
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Ethanol |
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Water |
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体内(现配现用): |
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<1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
制备储备液
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
1mM |
1.6016mL |
8.0079mL |
16.0159mL |
5mM |
0.3203mL |
1.6016mL |
3.2032mL |
10mM |
0.1602mL |
0.8008mL |
1.6016mL |
生物活性
产品描述 |
酸碱指示剂,pH变色范围6.0(黄色)-7.6(蓝色)。吸附指示剂。 |
靶点 |
Others |
体外研究 |
A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer.A pH-shift assay is developed on the basis of BTB as the pH indicator and potassium phosphate as the buffer component.Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate.The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5).Upon the addition of a Bromothymol Blue solution (0.1%,w/v) to an agar plate,zones surrounding colonies of K.vulgare 07 mutants change their color from blue to yellow because K.vulgare 07 mutants release 2-KLG on agar plates,thereby acidifying surrounding areas around colonies. |
推荐实验方法(仅供参考)
激酶实验: |
For all three enzymes,an expression in the 96-deep well scale is performed.Therefore,electrocompetent E.coli ArcticExpress(DE3) cells are transformed with the corresponding plasmid.Single clones are picked using the CP7200 Colony Picker and transferred to 96-deep well plates filled with 1.2mL autoinduction media by a MicroFlo Select dispenser.After incubation (36h,37℃ at 1,000 rpm),further processing is done manually. First,100μL of cell culture is transferred into a 96-well plate (U-shaped bottom) and harvested by centrifugation (4,570 rpm,10 min at RT) while the supernatant is discarded.The frozen pellets (1h at -20℃) are thawed at room temperature for one hour to improve cell lysis.Lysis is continued by the addition of 30μL lysis buffer (3h,1,000 rpm,37℃) containing 2mM KPi,pH 7.0,2mM MgCl2,10μg/mL DNaseI,100μg/mL lysozyme.Next,120μL buffer (2mM KPi,pH 7.0) is added followed by centrifugation (3,000 rpm,15 min at RT).For the photometric measurement,20μL of the crude extract is transferred to a 96-well plate (F-shaped bottom) and the reaction is started by adding 180μL master mix to give a final volume of 200μL (2.5mM KPi,pH 7.0,2mM MgCl2,25μg/mL BTB and 5mM keto-deoxy-D-glucarate).The measurements are carried out for 60 min at 2-min intervals.Depending on the enzyme,different time windows are used for the activity calculation. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )