| 中文名称: | 硫酸阿卡波糖 | ||||
|---|---|---|---|---|---|
| 英文名称: | Acarbose sulfate | ||||
| 别名: | 阿卡波糖硫酸盐 Acarbose sulfate | ||||
| CAS No: | 1221158-13-9 | 分子式: | C25H45NO22S | 分子量: | 743.68 |
| CAS No: | 1221158-13-9 | ||||
| 分子式: | C25H45NO22S | ||||
| 分子量: | 743.68 | ||||
基本信息
|
产品编号： |
A12365 |
||||
|
产品名称： |
Acarbose sulfate |
||||
|
CAS: |
1221158-13-9 |
储存条件 |
粉末 |
-20℃ |
四年 |
|
|
|
||||
|
分子式： |
溶于液体 |
|
|
||
|
分子量 |
743.68 |
|
|
||
|
化学名： |
|
||||
生物活性
|
产品描述 |
降糖药，是一种具有口服活性α-葡萄糖苷酶(alpha-glucosidase)抑制剂(IC50=11nM)。Acarbosesulfate能增强磺脲类药物或胰岛素的降血糖作用。 |
|
体外研究 |
Acarbose (1, 2, and 3μM) dose- and time-dependently inhibits TNF-α-induced VSMC proliferation and migration. Acarbose (1, 2, and 3μM) dose-dependently decreases β-galactosidase, Ras expression and increased p-AMPK expression in TNF-α pre-treated A7r5 cells |
|
体内研究 |
Acarbose (300mg/60 kg body weight) decreases the fasting blood glucose, and regulates the glucose tolerance of DM rats without body weight loss. Acarbose significantly suppresses serum IL6 and TNF-α in DM rats. Acarbose (2.5 and 5.0mg/kg) significantly and dose-dependently decreases the intensity of neointimal IL-6, TNF-α, and iNOS staining, and significantly increases the intensity of neointimal p-AMPK staining. Acarbose (2.5 and 5.0mg/kg) significantly and dose-dependently decreases neointimal Ras and β-galactosidase expression in HCD-fed rabbits without body weight loss |
推荐实验方法(仅供参考)
|
Cell Assay |
Cell viability is determined using the MTT assay. Cells are seeded in 24-well culture plates at a density of 2×104 cells/well, incubated for 48 h, treated with acarbose at varying concentrations (0.5, 1.0, 2.0, 3.0, and 5.0μM) for 24 h; or pre-treated with TNF-α (20ng/mL) for either 24 h or 48 h to evaluate the dose-dependent effects of acarbose on VSMC growth and viability, cultured with 0.5mg/mL MTT at 37°C in a humidified atmosphere of 5% CO2 for another 4 h, and solubilized with isopropanol. The viable cell number varies directly with the concentration of formazan product measured spectrophotometrically at 563nm |
|
|
|
|
Animal Administration |
Twenty-four male New Zealand white rabbits, weighing 2500 g are used. They are individually housed in metal cages in an air-conditioned room (22 ± 2°C, 55 ± 5% humidity), under a 12 h light/12 h dark cycle with free access to food and water. All rabbits are randomLy assigned to four groups of 6 animals each and are fed either standard chow (Group I), high cholesterol diet (HCD; containing 95.7% standard Purina chow + 3% lard oil + 0.5% cholesterol) (Group II), HCD diet and 2.5mg/kg per day acarbose (Group III), or HCD diet and 5.0mg/kg per day acarbose (Group IV). At the end of the 25 weeks, all rabbits are sacrificed by exsanguination under deep anesthesia with pentobarbital (30mg/kg i.v.) injected via the marginal ear vein. Serum is stored at −80°C prior to measurement of serum values. The aortic arch and thoracic aortas are carefully removed to protect the endothelial lining, and are collected and freed of adhering soft tissue. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
