中文名称: 腥红酸
英文名称: AMI-1 free acid
CAS No: 134-47-4
分子式: C21H16N2O9S2
分子量: 504.49
EINEC: 205-142-9
A11402 腥红酸 ≥98% (psaitong)
包装规格:
5g in glass bottle
溶解性:
溶于DMSO(83.33mg/mL 超声)
产品描述:

基本信息

产品编号:

A11402 

产品名称:

AMI-1 free acid

CAS:

134-47-4

 

储存条件

粉末

室温

四年

 

 

分子式:

C21H16N2O9S2

溶于液体

-80℃

两年

分子量

504.49

-20℃

1个月

化学名: 

7,7'-(Carbonylbis(azanediyl))bis(4-hydroxynaphthalene-2-sulfonic acid)

Solubility (25°C):

 

体外:

 

DMSO

20 mg/mL (39.64mM)

Ethanol

'-1 mg/mL

 

 

体内(现配现用):

 

1mg/ml表示微溶或不溶。

普西唐提供的所有化合物浓度为内部测试所得,实际溶液度可能与公布值有所偏差,属于正常的批间细微差异现象。

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;⼀旦配成溶液,请分装保存,避免反复冻融造成的产品失效。

 

制备储备液

 

浓度

 

溶液体积

质量

 

1mg

 

5mg

 

10mg

1mM

1.9822mL

9.9110mL

19.8220mL

5mM

0.3964mL

1.9822mL

3.9644mL

10mM

0.1982mL

0.9911mL

1.9822mL

 

生物活性

产品描述

一种有效的,细胞渗透性的,可逆的蛋白精氨酸 N-甲基转移酶 (PRMTs) 抑制剂,对人 PRMT1 和酵母 Hmt1p 作用的 IC50 值分别为 8.8μM 和 3.0μM。AMI-1 free acid 通过阻断肽-底物结合对 PRMTs 发挥抑制作用。

靶点

HMT 
()

human PRMT1 
()

yeast Hmt1p 
(Cell-free assay)

 

 

3.0μM

体外研究

AMI-1 free acid can inhibit the in vitro methylation reactions performed by all five recombinantly active PRMTs (PRMT1, -3, -4, and -6 and Hmt1p).
AMI-1 free acid not only inhibits type I PRMTs (PRMT1, 3, 4 and 6) but also type II PRMT5.
AMI-1 free acid specifically inhibits arginine, but not lysine, methyltransferase activity in vitro and does not compete for the AdoMet binding site.
AMI-1 free acid inhibits methylation of GFP-Npl3 and cellular proteins.
AMI-1 free acid (0.6-2.4mM; 48-96 hours) inhibits the cell viability of sarcoma in S180 and U2OS cells in a time-dependent and dose-dependent manner in vitro.
AMI-1 free acid (1.2-2.4mM; 48-72 hours) reduces S180 cell viability through the induction of cell apoptosis

 

Cell Viability Assay

Cell Line:

S180 cells, U2OS cells

Concentration:

0.6mM, 1.2mM, 2.4mM

Incubation Time:

48 hours, 72 hours, 96 hours

Result:

Inhibited the cell viability.

Apoptosis Analysis

Cell Line:

S180 cells

Concentration:

1.2mM, 2.4mM

Incubation Time:

48 hours, 72 hours

Result:

Increased the percentages of cells undergoing apoptosis.

体内研究

AMI-1 free acid (0.5 mg; intratumorally; daily; for 7 days) inhibits S180 viability in vivo.
AMI-1 free acid (0.5 mg; intratumorally; daily; for 7 days) downregulates PRMT5 but does not regulate the expression of PRMT7 in a tumor xenograft model.
AMI-1 free acid (0.5 mg; intratumorally; daily; for 7 days) decreases the levels of H4R3me2s and H3R8me2s in a tumor xenograft model

 

Animal Model:

6-7 weeks old male Kunming mice (18-22 g), with S180 cells xenograft

Dosage:

0.5 mg

Administration:

Intratumorally, daily, for 7 days

Result:

Decreased tumor weight.

保存条件:
室温
UN码:
HazardClass:
危害声明:
安全说明:
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参考文献 & 客户发表文献

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

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用本工具协助配置特定浓度的溶液,使用的计算公式为:
开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为:C1V1 = C2V2 ( 输入 输出 )

  • * = *

连续稀释计算器方程

  • 连续稀释

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  • 稀释倍数:
  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):