| 中文名称: | ANI-7 | ||||
|---|---|---|---|---|---|
| 英文名称: | ANI-7 | ||||
| CAS No: | 931417-26-4 | 分子式: | C13H8Cl2N2 | 分子量: | 263.12 |
| CAS No: | 931417-26-4 | ||||
| 分子式: | C13H8Cl2N2 | ||||
| 分子量: | 263.12 | ||||
基本信息
|
产品编号： |
A11362 |
||||
|
产品名称： |
ANI-7 |
||||
|
CAS: |
931417-26-4 |
储存条件 |
粉末 |
-20℃ |
四年 |
|
|
|
||||
|
分子式： |
溶于液体 |
-80℃ |
六个月 |
||
|
分子量 |
263.12 |
-20℃ |
一个月 |
||
|
化学名： |
(Z)-2-(3,4-dichlorophenyl)-3-(1H-pyrrol-2-yl)prop-2-enenitrile |
||||
|
Solubility (25°C)： |
|||||
|
体外:
|
DMSO |
20mg/mL(76.0109mM;Need ultrasonic) |
|||
|
Ethanol |
|
||||
|
Water |
|
||||
|
体内(现配现用): |
1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline，Solubility:≥2.08mg/mL(7.91mM);Clear solution |
||||
|
此⽅案可获得≥2.08mg/mL(7.91mM，饱和度未知)的澄清溶液。以1mL⼯作液为例，取100μL20.8mg/mL的澄清DMSO储备液加到400μLPEG300中，混合均匀；向上述体系中加⼊50μLTween-80，混合均匀；然后继续加⼊450μL⽣理盐⽔定容⾄1mL。 |
|||||
|
2.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.08mg/mL(7.91mM);Clear solution |
|||||
|
此⽅案可获得≥2.08mg/mL(7.91mM，饱和度未知)的澄清溶液，此⽅案不适⽤于实验周期在半个⽉以上的实验。以1mL⼯作液为例，取100μL20.8mg/mL的澄清DMSO储备液加到900μL⽟⽶油中，混合均匀。 |
|||||
|
＜1mg/ml表示微溶或不溶。 |
|||||
|
普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
|||||
|
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
|||||
制备储备液
|
浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
|
1mM |
3.8005mL |
19.0027mL |
38.0055mL |
|
5mM |
0.7601mL |
3.8005mL |
7.6011mL |
|
10mM |
0.3801mL |
1.9003mL |
3.8005mL |
生物活性
|
产品描述 |
一种AhR途径的激活剂。ANI-7抑制多种癌细胞的生长，并有选择地抑制MCF-7乳腺癌细胞的生长，GI50为0.56μM。ANI-7通过激活AhR途径诱导CYP1代谢单加氧酶，并在敏感的乳腺癌细胞系中诱导DNA损伤，检查点激酶(Chk2)激活，S期细胞周期停滞和细胞死亡。 |
|
|
靶点 |
Aryl Hydrocarbon Receptor |
Chk2 |
|
体外研究 |
ANI-7 (2.5μM; 24 hours; MCF10A and MDA-MB-468 cells) treatment induces significant S-phase and G2 + M-phase cell cycle arrest within 24 hours of treatment in MDA-MB-468 cells, and negligible effect in normal breast MCF10A cells[1] . ANI-7 (2μM; 12-24 hours; MDA-MB-468 cells) treatment results in a significant increase in the content and phosphorylation of CHK2, and induces a significant increase in H2AXɣ in MDA-MB-468 cells, indicative of DNA double-strand damage[1] . Inhibition of the AhR pathway ameliorates the effects of ANI-7. ANI-7 activates XRE activity and expression of the AhR and CYP1 members |
|
|
Comparisons of the GI50 values show that ANI-7 produces a GI50 value of 0.38μM in MCF-7 cells, whereas values of 3.0-42μM are observed in cell lines from lung, colon, ovary, neuronal, glial, prostate, and pancreas. The only other tumor type that shows appreciable growth inhibition by ANI-7 is the A431 vulva cell line (GI50 of 0.51μM)ANI-7 potently inhibits the growth of T47D, ZR-75-1, MCF-7, SKBR3, and MDA-MB-468 breast cancer cells (GI50 range of 0.16- 0.38μM), moderately inhibits the growth of BT20 and BT474 cells (GI50 range of 1-2μM), and essentially fails to inhibit the growth of MDA-MB-231 and MCF10A cells (GI50 range of 17-26μM). Moreover, ANI-7 maintained its ability to inhibit the growth of drug-resistant cells (MCF-7/VP16: GI50 of 0.21μM) Cell Cycle Analysis[1 |
||
|
Cell Line: |
MCF10A and MDA-MB-468 cells |
|
|
Concentration: |
2.5μM |
|
|
Incubation Time: |
24 hours |
|
|
Result: |
Induced significant S-phase and G2 + M-phase cell cycle arrest in MDA-MB-468 cells, and negligible effect in normal breast MCF10A cells. |
|
|
Western Blot Analysis |
||
|
Cell Line: |
MDA-MB-468 cells |
|
|
Concentration: |
2μM |
|
|
Incubation Time: |
12 hours, 24 hours |
|
|
Result: |
Resulted in a significant increase in the content and phosphorylation of CHK2 (25-fold increase),and induced a significant increase in H2AXɣ (3.5-fold increase). |
|
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
