| 中文名称: | 盐酸阿米洛利二水合物 | ||||
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| 英文名称: | Amiloride hydrochloride dihydrate | ||||
| 别名: | 盐酸阿米洛利二水合物 N-Amidino-3,5-diamino-6-chloropyrazinecarboxamide hydrochloride dihydrate | ||||
| CAS No: | 17440-83-4 | 分子式: | C6H8ClN7O.HCl.2H2O | 分子量: | 302.12 |
| CAS No: | 17440-83-4 | ||||
| 分子式: | C6H8ClN7O.HCl.2H2O | ||||
| 分子量: | 302.12 | ||||
基本信息
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产品编号： |
A11290 |
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产品名称： |
Amiloride hydrochloride dihydrate |
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CAS: |
17440-83-4 |
储存条件 |
粉末 |
2-8℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
302.12 |
-20℃ |
1个月 |
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化学名： |
3,5-diamino-N-carbamimidoyl-6-chloropyrazine-2-carboxamide hydrochloride dihydrate |
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Solubility (25°C)： |
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体外:
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DMSO |
100mg/mL (330.99mM) |
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Ethanol |
0mg/mL (0.0mM) |
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Water |
5mg/mL warmed with 50ºC water bath (16.54mM) |
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体内(现配现用): |
请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照In Vitro⽅式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐是指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶。 请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥2.5mg/mL(8.27mM);Clear solution 请依序添加每种溶剂：10% DMSO→90%(20% SBE-β-CD in saline) Solubility:≥2.5mg/mL(8.27mM);Clear solution 请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.5mg/mL(8.27mM);Clear solution |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
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1mM |
3.3099mL |
16.5497mL |
33.0994mL |
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5mM |
0.6620mL |
3.3099mL |
6.6199mL |
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10mM |
0.3310mL |
1.6550mL |
3.3099mL |
生物活性
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产品描述 |
是上皮钠通道(ENaC)和尿激酶型纤溶酶原激活物受体(uTPA)的抑制剂。 |
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靶点 |
ENaC;uTPA;polycystin-2(TRPP2) |
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体内研究 |
Amiloride hydrochloride dihydrate is a potent epithelial sodium channels (ENaCs) blocker.Amiloride is highly concentrated in the plasma 15 to 30 minutes following the injections.2mg/kg dose of Amiloride hydrochloride dihydrate has no effect on blood pressure,heart rate,mesenteric vascular resistance,or hindquarters vascular resistance as compare to the baseline measurements (n=7).Over a 2 hour period,Amiloride hydrochloride dihydrate elicits negligible responses in arterial pressure (-1±1 mmHg) and heart rate (-10±6 bpm/min) as compare to baseline levels.Results show an Amiloride hydrochloride dihydrate dose-related response pattern for the c-Fos activation in the area postrema (AP).Even at the lowest dose of Amiloride hydrochloride dihydrate (0.1mg/kg),the number of c-Fos labeled neurons in the AP is statistically different from the control rats at a p<0.01 level. |
推荐实验方法(仅供参考)
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动物实验: |
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The experiments are performed on male and female Sprague-Dawley rats that have been maintained in a heat controlled room maintained at 23℃ with a 12h/12h light-dark schedule.A total of 116 adult rats are injected i.p. with Amiloride hydrochloride dihydrate (0.1, 0.5, and 2.0mg/kg).After 15,30,45,or 120 min post-injection,the rats are anesthetized with 8% chloral hydrate,the chest is opened,and blood samples are obtained by cardiac puncture.The blood is centrifuged at 5000 RPM for 5 min,and the plasma (1.5mL) separated and stored at -80℃. |
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本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
