| 中文名称: | 盐酸阿米洛利 | ||||
|---|---|---|---|---|---|
| 英文名称: | Amiloride hydrochloride | ||||
| 别名: | 盐酸阿米洛利 N-Amidino-3,5-diamino-6-chloropyrazinecarboxamide monohydrochloride | ||||
| CAS No: | 2016-88-8 | 分子式: | C6H9Cl2N7O | 分子量: | 266.09 |
| CAS No: | 2016-88-8 | ||||
| 分子式: | C6H9Cl2N7O | ||||
| 分子量: | 266.09 | ||||
| EINEC: | 217-958-2 | ||||
| EINEC: | 217-958-2 | ||||
基本信息
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产品编号： |
A11271 |
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产品名称： |
Amiloride hydrochloride |
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CAS: |
2016-88-8 |
储存条件 |
粉末 |
2-8℃ |
四年 |
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分子式： |
溶于液体 |
-80℃ |
6个月 |
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分子量： |
266.09 |
-20℃ |
1个月 |
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化学名： |
3,5-diamino-6-chloro-N-(diaminomethylidene)pyrazine-2-carboxamide;hydrochloride |
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Solubility (25°C)： |
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体外:
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DMSO |
53mg/mL (199.18mM) |
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Ethanol |
5mg/mL (18.79mM) |
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Water |
6mg/mL (22.54mM) |
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体内(现配现用): |
请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照In Vitro⽅式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐是指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶。 1.请依序添加每种溶剂：10% DMSO→40% PEG300→5% Tween-80→45% saline Solubility:≥2.5mg/mL(9.40mM);Clear solution 2. 请依序添加每种溶剂：10% DMSO→90%(20% SBE-β-CD in saline) Solubility:≥2.5mg/mL(9.40mM);Clear solution 3.请依序添加每种溶剂：10% DMSO→90% corn oil Solubility:≥2.5mg/mL(9.40mM);Clear solution |
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＜1mg/ml表示微溶或不溶。 |
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普西唐提供的所有化合物浓度为内部测试所得，实际溶液度可能与公布值有所偏差，属于正常的批间细微差异现象。 |
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 |
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制备储备液
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浓度
溶液体积 质量 |
1mg |
5mg |
10mg |
|
1mM |
3.7581mL |
18.7906mL |
37.5813mL |
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5mM |
0.7516mL |
3.7581mL |
7.5163mL |
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10mM |
0.3758mL |
1.8791mL |
3.7581mL |
生物活性
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产品描述 |
是上皮钠通道(ENaC)和尿激酶型纤溶酶原激活物受体(uTPA)的抑制剂。Amiloride hydrochloride是polycystin-2(PC2;TRPP2)通道阻断剂。 |
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靶点 |
ENaC;uTPA;polycystin-2(TRPP2) |
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体外研究 |
Amiloride blocks δβγ channels with an IC50 of 2.6μM.The Ki of amiloride for δβγ ENaC is 26-fold that of αβγ channels (0.1μM for αβγ ENaC).Amiloride blockade of δβγ ENaC is much more voltage dependent compared with the αβγ channel.The Ki of amiloride for δαβγ channels is 920 and 13.7μM at −120 and +80 mV,respectively,which significantly differs from that of both αβγ and δβγ channels.Amiloride is a relatively selective inhibitor of the epithelial sodium channel (ENaC) with an IC50 (the concentration required to reach 50% inhibition of an ion channel) in the concentration range of 0.1 to 0.5μM.Amiloride is a relatively poor inhibitor of the the Na+/H+ exchanger (NHE) with an IC50 as low as 3μM in the presence of a low external [Na+] but as high as 1mM in the presence of a high [Na+].Amiloride is an even weaker inhibitor of the Na+/Ca2+ exchanger (NCX),with an IC50 of 1mM.Amiloride (1μM) and submicromolar doses of Benzamil (30nM),doses known to inhibit the ENaC,inhibit the myogenic vasoconstriction response to increasing perfusion pressure by blocking the activity of ENaC proteins.Amiloride completely inhibits Na+ influx in doses known to be relatively specific for ENaC (1.5μM) in vascular smooth muscle cells (VSMC). |
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体内研究 |
Amiloride (1mg/kg/day) subcutaneously is found to reverse the initial increases in collagen deposition and prevent any further increases in the DOCA-salt hypertensive rat.Amiloride delays the onset of proteinuria and improved brain and kidney histologic scores in the saline-drinking,stroke-prone spontaneously hypertensive rats (SHRSP) compared with controls.Amiloride antagonizes or prevents actions of aldosterone in these cells and in cardiovascular and renal tissues in animals with salt-dependent forms of hypertension. |
本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为：
质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)
摩尔浓度计算公式
用本工具协助配置特定浓度的溶液，使用的计算公式为：
开始浓度 x 开始体积 = 最终浓度 x 最终体积
稀释公式
稀释公式一般简略地表示为：C1V1 = C2V2 ( 输入 输出 )
